Murakami H, Kita K, Anraku Y
J Biol Chem. 1986 Jan 15;261(2):548-51.
A new b-type cytochrome, cytochrome b561 (Murakami, H., Kita, K., Oya, H., and Anraku, Y. (1984) Mol. Gen. Genet. 196, 1-5) was purified to near homogeneity from the cytochrome b561-amplified Escherichia coli K12 strain HM204/pAM5029. The purified cytochrome b561 was a single polypeptide with a molecular weight of 18,000, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its isoelectric point was determined to be 9.6. The difference spectrum of the cytochrome at 77 K shows a major alpha-absorption peak at 561 nm and a minor peak at 555 nm. The absolute spectrum at room temperature of the oxidized form of the cytochrome had an absorption peak at 414 nm, and that of the reduced form had peaks at 562, 530, and 428 nm. The oxidation-reduction potential of the cytochrome was estimated to be +20 mV. The cytochrome contained 91.2 nmol of heme/mg of protein, showing that it was a cytoplasmic membrane-bound, b-type diheme cytochrome.
一种新的b型细胞色素，细胞色素b561（村上浩、北川健、大矢浩和荒仓洋（1984年），《分子与普通遗传学》196卷，第1 - 5页）从细胞色素b561扩增的大肠杆菌K12菌株HM204/pAM5029中纯化至接近均一。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳测定，纯化的细胞色素b561是一种分子量为18,000的单一多肽。其等电点测定为9.6。该细胞色素在77 K时的差示光谱在561 nm处有一个主要的α吸收峰，在555 nm处有一个小峰。该细胞色素氧化形式在室温下的绝对光谱在414 nm处有一个吸收峰，还原形式在562、530和428 nm处有峰。该细胞色素的氧化还原电位估计为 +20 mV。该细胞色素每毫克蛋白质含有91.2 nmol的血红素，表明它是一种与细胞质膜结合的b型双血红素细胞色素。
