基于通用探针的侧向流动免疫分析的间接信号放大策略，用于快速定量检测伏马菌素 B1。

Indirect signal amplification strategy with a universal probe-based lateral flow immunoassay for the rapid quantitative detection of fumonisin B1.

机构信息

School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo 255049, Shandong Province, China.

Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo 255049, Shandong Province, China.

出版信息

Anal Methods. 2022 Feb 17;14(7):708-716. doi: 10.1039/d1ay01625c.

DOI:10.1039/d1ay01625c

PMID:35103722

Abstract

Fumonisin B1 (FB1) is a serious threat to the health of humans and animals. Herein, a lateral flow immunoassay based on universal detection probes (goat anti-mouse IgG@Eu) that could combine with any mouse monoclonal antibody was applied to detect FB1 in corn and feed. Compared with that based on direct monoclonal antibody labeling, this assay maintained bioactivity and saved consumption of monoclonal antibodies with the indirect signal amplification effect. The results indicated that this assay had higher sensitivity with a limit of detection (LOD) of 0.025 and 0.097 ng mL (0.50 and 1.94 ng g based on sample weight) in corn and feed, respectively. The detection range was about 1-50 ng mL (20-1000 ng g based on sample weight). In addition, the evaluation proved that it had good specificity, accuracy, precision, and applicability, and thus was suitable for the rapid and low-cost detection of fumonisin B1.

摘要

伏马菌素 B1（FB1）对人类和动物的健康构成严重威胁。本研究应用基于通用检测探针（羊抗鼠 IgG@Eu）的侧向流动免疫分析方法，该方法可与任何鼠单克隆抗体结合，用于检测玉米和饲料中的 FB1。与基于直接单克隆抗体标记的方法相比，该方法通过间接信号放大作用保持了生物活性并节省了单克隆抗体的用量。结果表明，该方法具有较高的灵敏度，在玉米和饲料中的检测限（LOD）分别为 0.025 和 0.097ng/mL（基于样品重量则分别为 0.50 和 1.94ng/g）。检测范围约为 1-50ng/mL（基于样品重量则分别为 20-1000ng/g）。此外，评估结果证明该方法具有良好的特异性、准确性、精密度和适用性，因此适用于伏马菌素 B1 的快速、低成本检测。