使用基于丝网印刷碳电极的免疫传感器测定伯利兹玉米制品中的伏马菌素B。

Fumonisin B Determination in Maize Products from Belize Using an Immunosensor Based on Screen-Printed Carbon Electrodes.

作者信息

Pérez-Fernández Beatriz, Maestroni Britt Marianna, Cozzani Carlotta, Eusey Colette, Gibson Natalie, de la Escosura-Muñiz Alfredo, Vlachou Christina

机构信息

NanoBioAnalysis Group, Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.

Food Safety and Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramerstrasse 5, A-1400 Vienna, Austria.

出版信息

Biosensors (Basel). 2025 Aug 12;15(8):526. doi: 10.3390/bios15080526.

DOI:10.3390/bios15080526

PMID:40862986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12384066/

Abstract

A competitive electrochemical immunosensor, using screen-printed carbon electrodes (SPCEs), was developed for the determination of total fumonisins (sum of FB1, FB2 and FB3) extracted with a simple solvent extraction and dilution method, without clean up, from maize flour and maize tortillas. The optimized biosensor has a linear range of 0.25 to 50 µg/L with 3% and 2% reproducibility for FB1 and (FB1 + FB2), respectively, and a linear range of 0.25 to 10 µg/L with 2% reproducibility for (FB1 + FB2 + FB3). The limits of detection and quantification in PBS buffer for total fumonisins are 0.12 µg/L and 0.39 µg/L, respectively. These values in the maize matrix are 6.07 µg/kg and 20.25 µg/kg, respectively. In addition, the stability and the selectivity of the sensor were studied. The immunosensor was validated with liquid chromatography-tandem mass spectrometry. This novel biosensor is more rapid, simpler and cheaper than current methods, and can also be used at the point of need.

摘要

一种采用丝网印刷碳电极（SPCEs）的竞争性电化学免疫传感器被开发出来，用于测定用简单溶剂萃取和稀释法从玉米粉和玉米饼中提取的总伏马菌素（FB1、FB2和FB3的总和），无需净化。优化后的生物传感器对FB1的线性范围为0.25至50 μg/L，重现性为3%，对（FB1 + FB2）的线性范围为0.25至50 μg/L，重现性为2%，对（FB1 + FB2 + FB3）的线性范围为0.25至10 μg/L，重现性为2%。在PBS缓冲液中，总伏马菌素的检测限和定量限分别为0.12 μg/L和0.39 μg/L。在玉米基质中的这些值分别为6.07 μg/kg和20.25 μg/kg。此外，还研究了该传感器的稳定性和选择性。该免疫传感器通过液相色谱-串联质谱法进行了验证。这种新型生物传感器比现有方法更快速、更简单、更便宜，并且也可在需要时使用。