Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea.
Department of Biomedical Science & Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Republic of Korea.
Int J Biol Macromol. 2022 Mar 15;201:686-697. doi: 10.1016/j.ijbiomac.2022.01.158. Epub 2022 Jan 29.
Recently, gold nanoparticles (AuNPs) are extensively used as peroxidase mimics. However, low catalytic activity, high synthesis cost, substrate-induced aggregation in reaction medium and difficulty in recovery and reuse still remain as major challenges. Here, a novel, simple, spontaneous, and reagent-less in-situ method for the production of AuNPs using dialdehyde cellulose nanofibrils (DACNF) is proposed. AuNPs synthesis time and size were greatly influenced by aldehyde content and the optimal aldehyde content for ultra-small AuNPs (≈10 nm) was 2.1 mM/g. AuNPs@DACNFs exhibited broad-spectrum peroxidase activity and steady-state kinetics revealed their better kinetic parameters (low K and high V) over horseradish peroxidase (HRP). AuNPs@DACNFs was further converted into paper strip, which served as a biosensor for HO and cholesterol detection. The proposed method exhibited wide linear response in the range of 10-90 μM and 0.05-0.45 mM, and detection limit of 0.39 μM and 1.9 μM for HO and cholesterol, respectively. Great shelf life and reusability were evident by FE-SEM and ICP-OES analysis. The smartphone application "Color Grab" was used to enable the portable onsite detection. The results of cholesterol detection in human serum samples were in agreement with clinically observed values, suggesting the great potential of the probe in disease diagnosis.
最近，金纳米粒子（AuNPs）被广泛用作过氧化物酶模拟物。然而，低催化活性、高合成成本、反应介质中底物诱导聚集以及回收和再利用困难仍然是主要挑战。本研究提出了一种新颖、简单、自发且无需试剂的原位方法，使用二醛纤维素纳米纤维（DACNF）生产 AuNPs。AuNPs 的合成时间和尺寸受醛含量的显著影响，最佳的超小 AuNPs（≈10nm）的醛含量为 2.1mM/g。AuNPs@DACNF 表现出广谱过氧化物酶活性，稳态动力学研究表明其比辣根过氧化物酶（HRP）具有更好的动力学参数（低 K 和高 V）。AuNPs@DACNF 进一步转化为纸条，可作为检测 HO 和胆固醇的生物传感器。该方法在 10-90μM 和 0.05-0.45mM 的范围内表现出宽线性响应，HO 和胆固醇的检测限分别为 0.39μM 和 1.9μM。通过 FE-SEM 和 ICP-OES 分析证明了其良好的保质期和可重复使用性。使用智能手机应用程序“Color Grab”可以实现便携式现场检测。人血清样本中胆固醇检测结果与临床观察值一致，表明该探针在疾病诊断中有很大的应用潜力。
