De Fazio S R, Hartner W C, Monaco A P, Gozzo J J
Transplantation. 1986 Jan;41(1):26-8. doi: 10.1097/00007890-198601000-00004.
Significant extended survival of C3H/He skin grafts in antilymphocyte serum (ALS)-treated B6AF1 mice can be brought about by the injection of donor-strain bone marrow on day 6 or 7 after grafting. In the present study, survival of the active graft-prolonging bone marrow cells under several storage conditions was investigated. The bone marrow cells retained their effectiveness if stored at 4 degrees C in 10% fetal calf serum for 18 hr prior to injection, but not if maintained at 37 degrees C under standard lymphocyte culture conditions. Freezing the cells for 10 days in a cryoprotective medium preserved the ability of the cells to prolong graft survival. In fact, freeze-thawed cells were more effective than fresh cells. Extension of the ALS-bone marrow treatment protocol to human transplantation is expected to be facilitated by frozen and short-term refrigerated storage of the donor bone marrow.
在抗淋巴细胞血清（ALS）处理的B6AF1小鼠中，C3H/He皮肤移植物显著延长存活时间可通过在移植后第6天或第7天注射供体品系骨髓实现。在本研究中，研究了活性移植物延长骨髓细胞在几种储存条件下的存活情况。如果在注射前于4℃在10%胎牛血清中储存18小时，骨髓细胞可保持其有效性，但在标准淋巴细胞培养条件下于37℃保存则不然。在冷冻保护培养基中冷冻细胞10天可保留细胞延长移植物存活的能力。事实上，冻融细胞比新鲜细胞更有效。供体骨髓的冷冻和短期冷藏保存有望促进ALS-骨髓治疗方案向人类移植的扩展。
