Tötterman T H, Nilsson K, Simonsson B
Br J Haematol. 1986 Jan;62(1):95-103. doi: 10.1111/j.1365-2141.1986.tb02904.x.
Earlier studies have indicated that serum beta 2m levels correlate with the disease activity, estimated tumour cell mass and prognosis of CLL. We have therefore analysed the spontaneous and phorbol ester (TPA)-induced capacity of CLL cells to produce beta 2m in vitro in relation to disease activity. Cell cultures (greater than 90% B cells) from 15 patients with active disease contained significantly higher (P less than 0.001) levels of beta 2m (0.22 +/- 0.09 mg/l) than cultures from 17 patients with inactive CLL (0.08 +/- 0.06). When TPA-induced CLL cell cultures were compared, this difference was even more striking (0.48 +/- 0.18 versus 0.13 +/- 0.09). The capacity of TPA-treated B-CLL cells to export beta 2m was positively correlated with their capacity to secrete monoclonal IgM (r = 0.73; P less than 0.001). Cell depletion experiments showed that the beta 2m export by B-CLL cells is enhanced by accessory T cells. Four individual CLL patients were followed for 14 months and repeatedly investigated for signs and symptoms of active disease, and the CLL cells were tested for in vitro production of beta 2m. Fluctuations in the clinical activity of the leukaemia were paralleled by similar switches in CLL cell production of beta 2m.
早期研究表明，血清β2微球蛋白（β2m）水平与慢性淋巴细胞白血病（CLL）的疾病活动度、估计的肿瘤细胞数量及预后相关。因此，我们分析了CLL细胞在体外自发产生及佛波酯（TPA）诱导产生β2m的能力与疾病活动度的关系。15例疾病活动期患者的细胞培养物（B细胞比例大于90%）中β2m水平（0.22±0.09mg/L）显著高于17例疾病非活动期CLL患者的培养物（0.08±0.06）（P<0.001）。比较TPA诱导的CLL细胞培养物时，这种差异更为显著（0.48±0.18对0.13±0.09）。经TPA处理的B-CLL细胞输出β2m的能力与其分泌单克隆IgM的能力呈正相关（r=0.73；P<0.001）。细胞清除实验表明，辅助性T细胞可增强B-CLL细胞的β2m输出。对4例CLL患者进行了14个月的随访，反复调查其疾病活动期的体征和症状，并检测CLL细胞体外产生β2m的情况。白血病临床活动度的波动与CLL细胞产生β2m的类似变化平行。
