Boettiger D, Dexter T M
Blood. 1986 Feb;67(2):398-405.
Long-term murine bone marrow cultures were used to support the growth and development of hematopoietic cells. After hematopoiesis was established, the cultures were infected with a recombinant murine amphotropic virus carrying the avian sarcoma virus src gene and the CFU-S kinetics were examined. The CFU-S from the src-infected cultures displayed a reduced seeding efficiency in the standard spleen colony assay. The self-renewal capacity of these CFU-S was tested by their ability to reestablish hematopoiesis when serially transplanted on irradiated bone marrow cultures and by serial passage in spleens of irradiated mice. In both tests, cells from the src-infected cultures exhibited an enhanced ability to sustain a high level of self-renewal. The other property of stem cells which may be measured is the probability of self-renewal at each cell division which dictates the distribution between stem cells and differentiated type progeny. CFU-S from the src-infected cultures had higher average probabilities of self-renewal and therefore reduced differentiation. These differences suggest that expression of src had indirectly or directly altered the normal differentiation program of the stem cells.
长期小鼠骨髓培养用于支持造血细胞的生长和发育。造血建立后，用携带禽肉瘤病毒src基因的重组鼠嗜双性病毒感染培养物，并检测CFU-S动力学。在标准脾集落试验中，来自src感染培养物的CFU-S显示接种效率降低。通过将这些CFU-S连续移植到经辐照的骨髓培养物上时重建造血的能力以及在经辐照小鼠脾脏中的连续传代来测试它们的自我更新能力。在这两种测试中，来自src感染培养物的细胞表现出增强的维持高水平自我更新的能力。可以测量的干细胞的另一个特性是每次细胞分裂时自我更新的概率，这决定了干细胞和分化型后代之间的分布。来自src感染培养物的CFU-S具有更高的平均自我更新概率，因此分化减少。这些差异表明src的表达间接或直接改变了干细胞的正常分化程序。
