Spooncer E, Boettiger D, Dexter T M
Nature. 1984;310(5974):228-30. doi: 10.1038/310228a0.
A molecular recombinant of Rous sarcoma virus and murine amphotropic leukaemia virus, src(MoMuLV), where the avian src oncogene has been placed under the influence of a murine virus promoter sequence, has been reported. Infection of long-term marrow cultures with this virus led to a dramatic change in the relative numbers of stem cells, granulocyte-macrophage progenitor cells and mature cells found in normal haematopoietic cell development. However, although the balance between self-renewal, differentiation and development was disturbed, injection of the cultured cells into irradiated syngeneic recipients did not lead to the development of leukaemia. Thus, although the control had been 'loosened', the host regulatory mechanisms were sufficient to impose a restraint on unlimited growth of the cells. We now show that the stem cells from the src-infected cultures show a remarkably increased capacity for self-renewal in vitro in situations which are inimical to the maintenance of self-renewal in normal uninfected stem cells and that self-renewal/differentiation can be modified by the culture conditions.
据报道，劳氏肉瘤病毒与小鼠嗜双性白血病病毒的一种分子重组体src（MoMuLV），其中禽类src癌基因已置于小鼠病毒启动子序列的影响之下。用这种病毒感染长期骨髓培养物，导致正常造血细胞发育中发现的干细胞、粒细胞-巨噬细胞祖细胞和成熟细胞的相对数量发生显著变化。然而，尽管自我更新、分化和发育之间的平衡受到干扰，但将培养细胞注射到经照射的同基因受体中并未导致白血病的发生。因此，尽管控制已被“放松”，宿主调节机制仍足以对细胞的无限生长施加限制。我们现在表明，来自src感染培养物的干细胞在体外显示出显著增强的自我更新能力，而这种情况对正常未感染干细胞的自我更新维持是不利的，并且自我更新/分化可被培养条件所改变。
