Carmena M J, Fernandez-Moreno M D, Prieto J C
Cell Biochem Funct. 1986 Jan;4(1):19-24. doi: 10.1002/cbf.290040103.
Insulin receptors have been characterized in rat prostatic epithelial cells by using [125I]insulin and a variety of physicochemical conditions. The binding data at equilibrium (2 h at 15 degrees C) could be interpreted in terms of two populations of insulin receptors: a class of receptors with high affinity (Kd = 2.16 nM) and low binding capacity (28.0 fmol mg-1 protein), and another class of receptors with low affinity (Kd = 0.29 microM) and high binding capacity (1.43 pmol mg-1 protein). Proinsulin exhibited a 63-fold lower affinity than insulin for binding sites whereas unrelated peptides were ineffective. The specific binding of insulin increased by about 50 per cent after 96 h of fasting; this increase could be explained by an increase of both the number of the high affinity-low capacity sites and the affinity of the low affinity-high capacity sites. These results together with previous studies on insulin action at the prostatic level strongly suggest that insulin may exert a physiological role on the prostatic epithelium.
通过使用[125I]胰岛素和各种物理化学条件，已在大鼠前列腺上皮细胞中对胰岛素受体进行了表征。平衡时（15℃下2小时）的结合数据可以用两种胰岛素受体群体来解释：一类具有高亲和力（Kd = 2.16 nM）和低结合能力（28.0 fmol mg-1蛋白质）的受体，以及另一类具有低亲和力（Kd = 0.29 microM）和高结合能力（1.43 pmol mg-1蛋白质）的受体。胰岛素原对结合位点的亲和力比胰岛素低63倍，而无关肽则无效。禁食96小时后，胰岛素的特异性结合增加了约50％；这种增加可以通过高亲和力-低容量位点数量的增加和低亲和力-高容量位点亲和力的增加来解释。这些结果与先前关于胰岛素在前列腺水平作用的研究一起强烈表明，胰岛素可能对前列腺上皮发挥生理作用。
