Tuberculosis Research Program at the Centenary Institute, The University of Sydney, Camperdown, Australia.
Victor Chang Cardiac Research Institute, Darlinghurst, Australia.
Zebrafish. 2022 Feb;19(1):7-17. doi: 10.1089/zeb.2021.0058. Epub 2022 Feb 2.
Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish and characterize the effects of and loss on embryonic development. We find that loss of or causes developmental defects in only a minority of embryos, in contrast to gene deletions in mice that cause loss of most embryos. Using a tail wound inflammation assay we find a conserved role for , but not , in normal macrophage migration to the wound site. Together our results indicate that zebrafish has clearly a partitioned role from that is more consistent with conserved functions of mammalian Heme oxygenase 1.
血红素加氧酶的功能在脊椎动物中高度保守，在正常胚胎发育中发挥重要作用，并控制氧化应激。已知斑马鱼血红素加氧酶 1 基因的表达对氧化应激有反应，表明其具有保守的生理功能。在这项研究中，我们生成了斑马鱼 的敲除等位基因，并研究了 和 缺失对胚胎发育的影响。我们发现，与小鼠中 基因缺失导致大多数胚胎丢失相比，缺失 或 仅导致少数胚胎发育缺陷。利用尾巴创伤炎症模型，我们发现 在正常巨噬细胞向创伤部位迁移中具有保守作用，但 没有。总之，我们的结果表明，斑马鱼 具有与哺乳动物血红素加氧酶 1 保守功能更一致的功能划分。
