Kakhniashvili D G, Smailov S K, Gavrilova L P
FEBS Lett. 1986 Feb 3;196(1):103-7. doi: 10.1016/0014-5793(86)80222-8.
The system of translation of Sepharose-bound poly(U) in which all ribosomes are active in peptide elongation was used to determine the stoichiometry of GTP hydrolysis at the stage of EF-Tu-promoted aminoacyl-tRNA binding. The ratio of GTP hydrolyzed at this stage per peptide bond was assayed during codon-specific elongation (polyphenylalanine synthesis) and misreading (polyleucine synthesis). It was demonstrated directly that the excess GTP hydrolyzed during misreading [(1984) FEBS Letters 178, 283-287] is expended at the stage of Ef-Tu-promoted binding of non-cognate aminoacyl-tRNA.
使用所有核糖体都在肽链延伸中起作用的琼脂糖结合多聚尿苷酸翻译系统，来确定在EF-Tu促进氨酰tRNA结合阶段GTP水解的化学计量。在密码子特异性延伸（多聚苯丙氨酸合成）和错读（多聚亮氨酸合成）过程中，测定了该阶段每形成一个肽键水解的GTP的比例。直接证明了错读过程中水解的过量GTP[（1984年）欧洲生物化学学会联合会快报178，283 - 287]是在Ef-Tu促进非同源氨酰tRNA结合阶段消耗的。
