Errick J E, Ing K W, Eggo M C, Burrow G N
In Vitro Cell Dev Biol. 1986 Jan;22(1):28-36. doi: 10.1007/BF02623438.
Human thyroid cells were grown and subcultured in vitro to examine their responses to known hormones and growth factors, and to serum. The cells were obtained from surgical specimens and were either neoplastic or nonneoplastic. The effects of culture conditions on cell growth were measured by changes in cell numbers and by stimulation of [3H]thymidine incorporation. The results showed that serum (0.5%) was essential for cell proliferation, and that a mixture of insulin (10 micrograms/ml), transferrin (5 micrograms/ml), hydrocortisone (10 micrograms/ml), somatostatin (10 ng/ml), and glycyl-histidyl-lysine (10 ng/ml) enhanced the effect of serum. Maximum growth of the cells was obtained when epidermal growth factor was present at 10(-9) M. Differentiation was measured by production of thyroglobulin, which was found to be stimulated by thyrotropin. This system provides a means to study the hormonal control of growth and differentiation in human thyroid cells.
将人甲状腺细胞在体外培养并传代培养，以研究它们对已知激素、生长因子及血清的反应。细胞取自手术标本，有肿瘤性的或非肿瘤性的。通过细胞数量的变化以及[3H]胸腺嘧啶核苷掺入的刺激来测量培养条件对细胞生长的影响。结果表明，0.5%的血清对细胞增殖至关重要，胰岛素（10微克/毫升）、转铁蛋白（5微克/毫升）、氢化可的松（10微克/毫升）、生长抑素（10纳克/毫升）和甘氨酰-组氨酰-赖氨酸（10纳克/毫升）的混合物可增强血清的作用。当表皮生长因子浓度为10^(-9) M时，细胞生长达到最大值。通过甲状腺球蛋白的产生来测量分化，发现促甲状腺激素可刺激甲状腺球蛋白的产生。该系统提供了一种研究人甲状腺细胞生长和分化的激素调控的方法。
