及其生物活性木犀草素对诱导性缺血再灌注后小鼠海马体的影响。

The Effect of and Its Bioactive Luteolin on the Hippocampus of Mice after Induced Ischemia Reperfusion.

作者信息

Mohammadpour Shahram, Ghiasyzadeh Fereshteh, Darvishi Marzieh, Karimi Elahe, Ghaneialvar Hori, Alizadeh Rafieh, Moayeri Ardeshir, Abbasi Naser

机构信息

Department of Anatomy, Medical School, Ilam University of Medical Sciences, Ilam, Iran.

Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.

出版信息

Evid Based Complement Alternat Med. 2022 Jan 24;2022:8157948. doi: 10.1155/2022/8157948. eCollection 2022.

DOI:10.1155/2022/8157948

PMID:35111230

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8803438/

Abstract

BACKGROUND

Ischemia plays an important role in increasing damage to the nervous system. This study aimed to evaluate the effect of (PFE) and its bioactive luteolin (Lu) and forced swimming exercise on the hippocampus of mice after induced ischemia reperfusion.

METHODS

The bioactive component of PFE (Lu) was identified by HPLC. Fifty-six male mice were divided into different groups. Ischemia was induced by ligation of the common carotid artery. After mice training (swimming exercise, 8 weeks) and consuming PFE and Lu, the mice's memory ability was evaluated in the shuttle box. Histological examination was performed by Nissel staining and immunohistochemistry.

RESULTS

Results showed that the ischemic mice exercised and treated with PFE and Lu had higher step-through latency (STL) compared with the nonexercised mice, and this was confirmed with time spent in the dark compartment (TDC). The number of dark cells in the ischemic group exercising and receiving PFE and Lu decreased compared to that of the other groups in the hippocampus. DCX protein expression was increased in nonexercised groups compared to that of the exercised groups and those treated with PFE and Lu, while NeuN decreased.

CONCLUSIONS

Forced swimming exercise following ischemia, as well as consumption of PFE and Lu, has reduced cell death and increased neurogenesis in the hippocampus and thus may help improve memory in ischemia.

摘要

背景

缺血在加重神经系统损伤方面起着重要作用。本研究旨在评估紫锥菊提取物（PFE）及其生物活性成分木犀草素（Lu）以及强迫游泳运动对诱导性缺血再灌注后小鼠海马体的影响。

方法

通过高效液相色谱法鉴定PFE的生物活性成分（Lu）。将56只雄性小鼠分为不同组。通过结扎颈总动脉诱导缺血。在小鼠训练（游泳运动，8周）并摄入PFE和Lu后，在穿梭箱中评估小鼠的记忆能力。通过尼氏染色和免疫组织化学进行组织学检查。

结果

结果显示，与未运动的小鼠相比，进行运动并接受PFE和Lu治疗的缺血小鼠具有更高的穿梭潜伏期（STL），这在暗箱停留时间（TDC）中得到证实。与其他组相比，运动并接受PFE和Lu治疗的缺血组海马体中暗细胞数量减少。与运动组以及接受PFE和Lu治疗的组相比，未运动组中双皮质素（DCX）蛋白表达增加，而神经元核抗原（NeuN）减少。