从昆虫病原线虫 Steinernema ceratophorum 中分离到一种新型二分病毒的证据。
Evidence for a novel partitivirus isolated from the entomopathogenic nematode Steinernema ceratophorum.
机构信息
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
出版信息
Arch Virol. 2022 Mar;167(3):969-972. doi: 10.1007/s00705-021-05314-5. Epub 2022 Feb 3.
Nematodes are abundant, but little is known about their viruses. In this study, we report a novel partitivirus isolated from the entomopathogenic nematode species Steinernema ceratophorum, named "Steinernema ceratophorum partitivirus 1" (ScPV-1). The complete genome of ScPV-1 comprises two dsRNA segments, dsRNA1 (2352 bp) and dsRNA2 (2196 bp). Each dsRNA contains a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. The sequences of the RdRp and CP showed the highest similarity (47% and 33% identity, respectively) to Plasmopara viticola associated partitivirus 7 (PvAP-7). A multiple sequence alignment and phylogenetic analysis of the RdRp of ScPV-1 and other selected viruses indicated that ScPV-1 is a new member of the genus Betapartitivirus in the family Partitiviridae.
线虫数量丰富，但对其病毒知之甚少。在这项研究中，我们报告了一种从昆虫病原线虫 Steinernema ceratophorum 中分离出来的新型二分体病毒，命名为“Steinernema ceratophorum partitivirus 1”（ScPV-1）。ScPV-1 的完整基因组由两条 dsRNA 片段组成，dsRNA1（2352bp）和 dsRNA2（2196bp）。每个 dsRNA 都包含一个单一的开放阅读框（ORF），分别编码一个假定的 RNA 依赖性 RNA 聚合酶（RdRp）和外壳蛋白（CP）。RdRp 和 CP 的序列与 Plasmopara viticola 相关的二分体病毒 7（PvAP-7）显示出最高的相似性（分别为 47%和 33%的同一性）。ScPV-1 和其他选定病毒的 RdRp 的多序列比对和系统发育分析表明，ScPV-1 是二分体病毒科二分体病毒属的一个新成员。
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