Department of Urology, Area 3, Tangshan Gongren Hospital, LubeiDistrict, No.27 Wenhua Road, Tangshan, Hebei, 063000, People's Republic of China.
Environ Sci Pollut Res Int. 2022 Jun;29(26):39985-39993. doi: 10.1007/s11356-021-18343-z. Epub 2022 Feb 3.
Renal cell carcinoma (RCC) is a highly recurrent aggressive tumor. This study works for the regulation of miR-21-5p on RCC cell functions and novel ideas for therapies of RCC. Isoform expression quantification data were offered by The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA-KIRC) to investigate differentially expressed miRNAs. The way miR-21-5p works on biological functions of RCC was examined with MTT and Transwell assays. The downstream targets of miR-21-5p were predicted using bioinformatics analysis. The binding of two researched objects was verified by the dual-luciferase method. TCGA data manifested a considerably high level of miR-21-5p in RCC tissue, while ARHGAP24 was significantly lowly expressed. miR-21-5p bound ARHGAP24 and stimulated RCC cell functions, whereas ARHGAP24 mimic could reverse such promotion. This work observed miR-21-5p, a stimulator in RCC, and it deteriorated this cancer via repressing its downstream target gene ARHGAP24 expression.
肾细胞癌（RCC）是一种高度复发的侵袭性肿瘤。本研究旨在探讨 miR-21-5p 对 RCC 细胞功能的调节作用，为 RCC 的治疗提供新的思路。本研究利用癌症基因组图谱肾透明细胞癌（TCGA-KIRC）提供的异构体表达定量数据，探讨差异表达的 miRNAs。通过 MTT 和 Transwell 检测分析 miR-21-5p 对 RCC 细胞生物学功能的影响。利用生物信息学分析预测 miR-21-5p 的下游靶基因。采用双荧光素酶报告基因实验验证两个研究对象的结合。TCGA 数据显示 RCC 组织中 miR-21-5p 水平显著升高，而 ARHGAP24 表达明显降低。miR-21-5p 与 ARHGAP24 结合并刺激 RCC 细胞功能，而 ARHGAP24 模拟物可以逆转这种促进作用。本研究观察到 miR-21-5p 作为 RCC 的一种刺激物，通过抑制其下游靶基因 ARHGAP24 的表达，恶化了这种癌症。
