Enzymatic biodegradation, kinetic study, and detoxification of Reactive Red-195 by isolated from Marine Sediments of Andaman Sea, India.

Azo dyes are a significant class of hazardous chemicals that are extensively utilised in diverse industries. Industries that manufacture and consume reactive azo dyes generate hyper-saline wastewater. The ability of halotolerant bacteria to thrive under extreme environmental conditions thus makes them a potential candidate for reactive azo dye degradation. An efficient halotolerant bacterium (isolate SAIBP-6) with the capability to degrade 87.15% of azo dye Reactive Red 195 (RR-195) was isolated from sea sediment and identified as SAIBP-6. Strain SAIBP-6 maintained potential decolourisation under a wide range of environmental conditions viz. 35-45°C temperature, 50-450 mg/L RR-195, pH 7-9, and 50-150 g/L NaCl. However, maximum decolourisation occurred at 40°C, 200 mg/L RR-195 dye, pH 9, and 50 g/L NaCl, under static conditions. Tyrosinase and azoreductase were responsible for dye degradation. The reaction catalysed by these enzymes followed zero-order kinetics. The maximum velocity () of the enzymatic reaction was 4.221 mg/(L.h) and the Michaelis constant () was 517.982 mg/L. Strain SAIBP-6 also efficiently decolourised Reactive Black-5 and Reactive Yellow-160 dye. The biodegradation process was further studied with the help of UV-Vis spectral scan, ultra-high performance liquid chromatography (UPLC), fourier-transform infra-red spectroscopy (FT-IR), and proton nuclear magnetic resonance (H NMR) analysis. Finally, cytogenotoxicity assay conducted with the meristematic root tip cells of and phytotoxicity assay conducted with the seeds of led to the inference that strain SAIBP-6 significantly reduced the toxicity of RR-195 after biodegradation.