Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar (A Central) University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226 025, India; CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, 440 020, Maharashtra, India.
Aquatic Toxicology Laboratory, Environmental Toxicology Group, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, M.G. Marg, Lucknow, 226 001, Uttar Pradesh, India.
Chemosphere. 2022 May;295:133823. doi: 10.1016/j.chemosphere.2022.133823. Epub 2022 Feb 1.
Heavy metals phytoremediation from pulp and paper industry (PPI) sludge was conducted by employing root-associated Brevundimonas sp (PS-4 MN238722.1) in rhizospheric zone of Saccharum munja L. for its detoxification. The study was aimed to investigate the efficiency of Saccharum munja L. for the removal of heavy metals along with physico-chemical parameters through bacterial interactions. Physico-chemical examination of PPI sludge showed biochemical oxygen demand (8357 ± 94 mg kg), electrical conductivity (2264 ± 49 μmhoscm), total phenol (521 ± 24 mg kg), total dissolve solid (1547 ± 23 mg kg), total nitrogen (264 ± 2.13 mg kg), pH (8.2 ± 0.11), chemical oxygen demand (34756 ± 214 mg kg), color (2434 ± 45 Co-Pt), total suspended solid (76 ± 0.67 mg kg), sulphate (2462 ± 13 mg kg), chlorolignin (597 ± 13.01 mg kg), K (21.04 ± 0.26 mg kg), total solid (1740 ± 54 mg kg), phosphorous, Cl, and Na. Heavy metals, such as Fe followed by Zn, Mn, Cd, Cu, Ni, Pb, As, Cr and Hg were above the permissible limit. Root and shoot of Saccharum munja L. revealed highest concentrations of Cd followed by Mn, Ni, Fe, Zn, Cu, As, Cr, Hg, and Pb. Tested metals (Fe, Mn, Pb, Cd, Cr, Cu, Zn, Ni, As, and Hg) bioaccumulation and translocation factors were also revealed to be < 1 and >1, respectively, demonstrating that these plants have considerable absorption and translocation abilities. Plant growth-promoting activity, such as ligninolytic enzymes, hydrolytic enzymes, indole acetic acid, and siderophore production activity of Brevundimonas sp. (PS-4 MN238722.1) were also noted to be higher. These findings support the use of Brevundimonas sp (PS-4 MN238722.1) in combination with Saccharum munja L. plant as interdisciplinary management of industrial sludge at polluted areas for the prevention of soils near the industrial site.
采用根际 Brevundimonas sp（PS-4 MN238722.1）对制浆造纸厂（PPI）污泥进行重金属植物修复，以实现其解毒。本研究旨在通过细菌相互作用，研究甘蔗（Saccharum munja L.）对重金属的去除效率及其与理化参数的关系。PPI 污泥的理化性质检测表明，生化需氧量（8357±94mgkg）、电导率（2264±49μmhoscm）、总酚（521±24mgkg）、总溶解固体（1547±23mgkg）、总氮（264±2.13mgkg）、pH 值（8.2±0.11）、化学需氧量（34756±214mgkg）、颜色（2434±45 Co-Pt）、总悬浮固体（76±0.67mgkg）、硫酸盐（2462±13mgkg）、氯代木质素（597±13.01mgkg）、K（21.04±0.26mgkg）、总固体（1740±54mgkg）、磷、Cl 和 Na。铁、锌、锰、镉、铜、镍、铅、砷、铬和汞等重金属含量均超过允许限值。甘蔗的根和茎中镉的浓度最高，其次是锰、镍、铁、锌、铜、砷、铬、汞和铅。测试的金属（铁、锰、铅、镉、铬、铜、锌、镍、砷和汞）的生物积累和迁移因子也分别显示为 <1 和 >1，表明这些植物具有相当高的吸收和迁移能力。还注意到 Brevundimonas sp.（PS-4 MN238722.1）的植物促生活性，如木质素分解酶、水解酶、吲哚乙酸和铁载体的产生活性也较高。这些发现支持将 Brevundimonas sp.（PS-4 MN238722.1）与甘蔗（Saccharum munja L.）植物联合使用，作为受污染地区工业污泥的跨学科管理，以防止工业场地附近土壤的污染。
