Li Jun, Du Cuiwei, Liu Zhiyong, Li Xiaogang
Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China; Key Laboratory for Corrosion and Protection of The Ministry of Education (MOE), Beijing 100083, China.
Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China; Key Laboratory for Corrosion and Protection of The Ministry of Education (MOE), Beijing 100083, China; Beijing Advanced Innovation Center for Materials Genome Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Bioelectrochemistry. 2022 Jun;145:108074. doi: 10.1016/j.bioelechem.2022.108074. Epub 2022 Jan 21.
Bacillus licheniformis can take up electrons from X80 steel for nitrate reduction and accelerate corrosion. In this work, the routes B. licheniformis adopts for extracellular electron transfer (EET) were revealed using electrochemical tests, fluorescence staining, high performance liquid chromatography (HPLC) and weight loss tests, and their kinetics were also analyzed in detail. The results showed that B. licheniformis can take up electrons from steel surface via three routes: direct electron transfer by cytochromes, direct electron transfer by flavin-bound cytochromes and mediated electron transfer by diffusible flavin. B. licheniformis itself can only secrete traces of riboflavin which are mainly bound to the surface cytochromes assisting electron transfer. Adding flavins can increase the bound content, and with the binding sites of cytochrome becoming saturated the rest of flavins will mediate electron transfer through diffusion even more efficiently than the bound.
地衣芽孢杆菌可以从X80钢中获取电子用于硝酸盐还原并加速腐蚀。在本研究中，通过电化学测试、荧光染色、高效液相色谱（HPLC）和失重测试揭示了地衣芽孢杆菌进行胞外电子转移（EET）的途径，并对其动力学进行了详细分析。结果表明，地衣芽孢杆菌可通过三种途径从钢表面获取电子：细胞色素介导的直接电子转移、黄素结合细胞色素介导的直接电子转移以及可扩散黄素介导的电子转移。地衣芽孢杆菌自身仅分泌痕量核黄素，这些核黄素主要与表面细胞色素结合以辅助电子转移。添加黄素可增加结合量，随着细胞色素的结合位点饱和，其余黄素将通过扩散介导电子转移，且比结合态的更高效。
