Marak Chuckles Ch, Marak Brilliant N, Singh Ved Prakash, Gurusubramanian Guruswami, Roy Vikas Kumar
Department of Zoology, Mizoram University, Aizawl, India.
Department of Industrial Chemistry, Mizoram University, Aizawl, India.
Drug Chem Toxicol. 2023 Mar;46(2):330-342. doi: 10.1080/01480545.2022.2033258. Epub 2022 Feb 3.
Fruit of Buch.-Ham has been used as medicine by the local community in some parts of the north eastern state of India. Despite its uses for different purposes, the safety assessment study has not been conducted. Therefore, we have evaluated the acute and the sub-acute toxicity of methanolic extract of fruit (CPFE) in a mice model via oral route of administration. Phytochemicals analysis was carried out by liquid chromatography-mass spectroscopy (LC-MS), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FTIR). The acute toxicity study was performed at a single dose of 1000, 3000 and 5000 mg/kg and the sub-acute toxicity study at a dose of 100, 300 and 500 mg/kg was administered daily for 28 days. The calculated Lethal dose 50 (LD50) of CPFE was found to be 4000 mg/kg. Both acute and sub-acute studies showed that 5000 mg/kg and 500 mg/kg dose was toxic to the mice. The results of acute toxicity showed CPFE could have a mild toxic effect on the kidney at a dose of 3000 and 5000 mg/kg, as some deteriorated changes in the kidney along with increase creatinine levels were observed. Acute toxicity also showed an increase in white blood cells (WBC) at a dose of 3000 mg/kg.However, sub-acute toxicity studies do not show any detrimental effects on liver, kidney and hematological parameters. Thus, it can be suggested that CPFE at a dose of 100 and 300 mg/kg would be safe for consumption. The phytochemicals analysis by LC-MS, NMR and FTIR showed the presence of 32 major chemical compounds with certain biological activity like anti-neoplastic, antioxidant, and possible modulator of steroid metabolism (cholesterol antagonist and agonist of testosterone 17β-dehydrogenase) as predicted by PASS analysis.
在印度东北部某邦的一些地区，当地社区一直将布坎南氏（Buch.-Ham）果实入药。尽管其有多种用途，但尚未开展安全性评估研究。因此，我们通过口服给药途径，在小鼠模型中评估了果实甲醇提取物（CPFE）的急性和亚急性毒性。采用液相色谱 - 质谱联用（LC-MS）、核磁共振（NMR）和傅里叶变换红外光谱（FTIR）进行了植物化学分析。急性毒性研究采用1000、3000和5000 mg/kg的单一剂量，亚急性毒性研究采用100、300和500 mg/kg的剂量，每日给药，持续28天。发现CPFE的半数致死剂量（LD50）为4000 mg/kg。急性和亚急性研究均表明，5000 mg/kg和500 mg/kg的剂量对小鼠有毒。急性毒性结果显示，在3000和5000 mg/kg剂量下，CPFE可能对肾脏有轻度毒性作用，因为观察到肾脏出现一些恶化变化以及肌酐水平升高。急性毒性还显示，在3000 mg/kg剂量下白细胞（WBC）增加。然而，亚急性毒性研究未显示对肝脏、肾脏和血液学参数有任何有害影响。因此，可以认为100和300 mg/kg剂量的CPFE食用是安全的。通过LC-MS、NMR和FTIR进行的植物化学分析表明，存在32种主要化合物，具有某些生物活性，如抗肿瘤、抗氧化以及如PASS分析所预测的可能的类固醇代谢调节剂（胆固醇拮抗剂和睾酮17β - 脱氢酶激动剂）。
