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苦味受体通过抑制细胞衰老和促进伤口愈合来防止皮肤衰老。

Bitter taste receptors protect against skin aging by inhibiting cellular senescence and enhancing wound healing.

作者信息

Chung Min Gi, Kim Yerin, Cha Yeon Kyung, Park Tai Hyun, Kim Yuri

机构信息

Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea.

Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Korea.

出版信息

Nutr Res Pract. 2022 Feb;16(1):1-13. doi: 10.4162/nrp.2022.16.1.1. Epub 2021 May 25.

DOI:10.4162/nrp.2022.16.1.1
PMID:35116124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8784259/
Abstract

BACKGROUND/OBJECTIVES: Bitter taste receptors are taste signaling pathway mediators, and are also expressed and function in extra-gustatory organs. Skin aging affects the quality of life and may lead to medical issues. The purpose of this study was to better understand the anti-skin aging effects of bitter taste receptors in D-galactose (D-gal)-induced aged human keratinocytes, HaCaT cells.

MATERIALS/METHODS: Expressions of bitter taste receptors in HaCaT cells and mouse skin tissues were examined by polymerase chain reaction assay. Bitter taste receptor was overexpressed in HaCaT cells, and D-gal was treated to induce aging. We examined the effects of bitter taste receptors on aging by using β-galactosidase assay, wound healing assay, and Western blot assay.

RESULTS

TAS2R16 and TAS2R10 were expressed in HaCaT cells and were upregulated by D-gal treatment. TAS2R16 exerted protective effects against skin aging by regulating p53 and p21, antioxidant enzymes, the SIRT1/mechanistic target of rapamycin pathway, cell migration, and epithelial-mesenchymal transition markers. TAS2R10 was further examined to confirm a role of TAS2R16 in cellular senescence and wound healing in D-gal-induced aged HaCaT cells.

CONCLUSIONS

Our results suggest a novel potential preventive role of these receptors on skin aging by regulating cellular senescence and wound healing in human keratinocyte, HaCaT.

摘要

背景/目的：苦味受体是味觉信号通路的介质，也在味觉外器官中表达并发挥作用。皮肤老化影响生活质量，并可能导致医学问题。本研究的目的是更好地了解苦味受体在D-半乳糖（D-gal）诱导的人角质形成细胞HaCaT细胞皮肤老化中的抗皮肤老化作用。

材料/方法：通过聚合酶链反应检测法检测HaCaT细胞和小鼠皮肤组织中苦味受体的表达。在HaCaT细胞中过表达苦味受体，并用D-gal处理诱导老化。我们通过β-半乳糖苷酶检测法、伤口愈合检测法和蛋白质印迹检测法研究了苦味受体对老化的影响。

结果

TAS2R16和TAS2R10在HaCaT细胞中表达，并在D-gal处理后上调。TAS2R16通过调节p53和p21、抗氧化酶、SIRT1/雷帕霉素作用靶点通路、细胞迁移和上皮-间质转化标志物发挥抗皮肤老化作用。进一步检测TAS2R10以证实TAS2R16在D-gal诱导的老化HaCaT细胞的细胞衰老和伤口愈合中的作用。

结论

我们的结果表明，这些受体通过调节人角质形成细胞HaCaT中的细胞衰老和伤口愈合，对皮肤老化具有新的潜在预防作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/647f02af00bd/nrp-16-1-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/af91792bdbeb/nrp-16-1-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/d709015db212/nrp-16-1-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/2a8747649f32/nrp-16-1-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/647f02af00bd/nrp-16-1-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/af91792bdbeb/nrp-16-1-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/d709015db212/nrp-16-1-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/2a8747649f32/nrp-16-1-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/568a/8784259/647f02af00bd/nrp-16-1-g004.jpg

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