Song Xiaodong, Shao Zili, Han Menglin, Liang Huihong
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China.
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
Transl Cancer Res. 2019 Apr;8(2):583-591. doi: 10.21037/tcr.2019.03.19.
Cholangiocarcinoma (CCA) is the second most common primary hepatic malignancy in humans. Although early-stage CCA can be managed with surgery, CCA is considered incurable at advanced stages and results in poor quality of life and overall survival. A good orthotopic CCA animal model is essential to perform basic studies investigating CCA in order to understand the molecular pathways that underlie cancer development, and to develop new therapies for the prevention and treatment of CCA. However, to the best of our knowledge, orthotopic extrahepatic CCA animal models have not yet been reported in the literature.
In this study, we established an orthotopic extrahepatic CCA model in mice using a two-step surgical procedure.
The characteristics of this model were confirmed using molecular imaging and histological analysis.
We believe that this CCA animal model, which can be established quickly, easily, and with good reproducibility, will help guide research on novel diagnostic and treatment strategies for extrahepatic CCA.
胆管癌（CCA）是人类第二常见的原发性肝脏恶性肿瘤。尽管早期CCA可通过手术治疗，但晚期CCA被认为无法治愈，会导致生活质量差和总体生存期缩短。一个良好的原位CCA动物模型对于开展研究CCA的基础研究至关重要，以便了解癌症发生发展的分子途径，并开发预防和治疗CCA的新疗法。然而，据我们所知，文献中尚未报道过原位肝外CCA动物模型。
在本研究中，我们采用两步手术程序在小鼠中建立了原位肝外CCA模型。
使用分子成像和组织学分析证实了该模型的特征。
我们认为，这种能够快速、轻松建立且具有良好重复性的CCA动物模型，将有助于指导肝外CCA新型诊断和治疗策略的研究。
