BACKGROUND

This study aimed to investigate the regulatory role of () in the progression of acute myeloid leukemia (AML) and the underlying mechanism.

METHODS

expression was detected in AML cell lines AML-193, KG-1, HL-60, OCI-AML2 and primary normal bone marrow mononuclear cells (BMMC). The effect of knockdown on cell proliferation, apoptosis and expression were detected by transfection of shRNA and control shRNA into KG-1 cells. And the effect of knockdown on , cell proliferation, cell apoptosis as well as potential target genes [ () and ()] in knockdown treated KG-1 cells was assessed by transfection of shRNA and shRNA & shRNA into KG-1 cells.

RESULTS

expression was elevated in AML-193, KG-1, OCI-AML2 cell lines, but similar in HL-60 cell line compared with primary normal BMMC. knockdown inhibited cell proliferation but promoted cell apoptosis in KG-1 cells, meanwhile expression was increased by knockdown in KG-1 cells. And in rescue experiments, knockdown had no effect on expression of , while it increased the expressions of and , promoted cell proliferation, inhibited cell apoptosis in knockdown treated KG-1 cells.

CONCLUSIONS

knockdown inhibits cell proliferation but promotes cell apoptosis via regulating mediated and in AML.