Jiang Xiaoping, Chen Kai, Fan Kaixi, Guo Jing
Department of Respiratory, Shanxian Central Hospital, Heze 274300, China.
Department of Cardiothoracic Surgery, The 2nd Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325027, China.
Transl Cancer Res. 2020 Jan;9(1):12-20. doi: 10.21037/tcr.2019.11.13.
Abundant evidence suggests that a neurotrophic factor, artemin (ARTN), is involved in the tumorigenesis and progression in several malignancies. However, the biological functions of ARTN in gastric cancer (GC) remain poorly elucidated.
ARTN expression was evaluated by immunohistochemistry in GC tissue, and its clinical and prognosis significance was analyzed. Cell counting kit-8 (CCK-8), transwell chamber assay, and western blot were used to detect the effects of ARTN knockdown on GC cell behavior .
ARTN was highly expressed in GC tissue, and its positive expression predicted poor prognosis of GC. studies showed that ARTN knockdown inhibited the STAT3 phosphorylation, thus impeding cell proliferation and DNA synthesis in GC. Furthermore, the promotion of ARTN on the migration and invasion of GC cells was achieved by regulating the expression of MMP9 and E-cadherin.
ARTN might be a promising prognostic marker and a potential therapeutic target for GC.
大量证据表明，神经营养因子Artemin（ARTN）参与了多种恶性肿瘤的发生和发展。然而，ARTN在胃癌（GC）中的生物学功能仍不清楚。
采用免疫组织化学方法检测GC组织中ARTN的表达，并分析其临床及预后意义。使用细胞计数试剂盒-8（CCK-8）、Transwell小室实验和蛋白质免疫印迹法检测敲低ARTN对GC细胞行为的影响。
ARTN在GC组织中高表达，其阳性表达预示着GC的预后不良。研究表明，敲低ARTN可抑制STAT3磷酸化，从而阻碍GC细胞增殖和DNA合成。此外，ARTN通过调节MMP9和E-钙黏蛋白的表达促进GC细胞迁移和侵袭。
ARTN可能是一种有前景的GC预后标志物和潜在治疗靶点。
