结肠癌中CNN2的异位表达促进细胞迁移。
Ectopic expression of CNN2 of colon cancer promotes cell migration.
作者信息
Zheng Lei, Zhao Peng, Liu Kai, Kong Da-Lu
机构信息
Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
出版信息
Transl Cancer Res. 2020 Feb;9(2):1063-1069. doi: 10.21037/tcr.2019.12.61.
BACKGROUND
Calponin is an actin filament-associated regulatory protein originally identified in smooth muscle cells. Three homologous have been identified in vertebrate species, but little functional characterization of potential activities in cancer has been performed. In this study, we determined the levels of CNN2 in colon cancer cell lines and determined the effects of this protein by increasing expression.
METHODS
We used IHC and RT-PCR to measure CNN2 expression in colon cancer tissues and normal tissues and found increased expression levels in cancer tissues. We used viral vectors to decrease the level of CNN2 in the SW480 colon cancer cell line and found that silencing of CNN2 inhibited cell invasion. We detected potential protein interactions in signal pathways by western bolt analysis. To confirm the effect of CNN2 on cell invasion, we increased CNN2 expression in the SW620 cell line and observed increased invasion increased expression levels of associated proteins.
RESULTS
High-expression levels of CNN2 were detected in cancer tissues. Silencing CNN2 inhibited cell invasion, down-regulated N-cadherin (N-CA) and C-myc proteins, and up-regulated E-cadherin (E-CA), suggesting that CNN2 promotes colon cancer cell invasion. Increasing CNN2 levels in the SW620 cell line showed the opposite results.
CONCLUSIONS
CNN2 may act to promote colon cancer.
背景
钙调蛋白是一种最初在平滑肌细胞中发现的与肌动蛋白丝相关的调节蛋白。在脊椎动物物种中已鉴定出三种同源物，但对其在癌症中潜在活性的功能表征却很少。在本研究中，我们测定了结肠癌细胞系中CNN2的水平，并通过增加其表达来确定该蛋白的作用。
方法
我们使用免疫组织化学（IHC）和逆转录-聚合酶链反应（RT-PCR）来测量结肠癌组织和正常组织中CNN2的表达，发现癌组织中的表达水平升高。我们使用病毒载体降低SW480结肠癌细胞系中CNN2的水平，发现沉默CNN2可抑制细胞侵袭。我们通过蛋白质免疫印迹分析检测信号通路中潜在的蛋白质相互作用。为了证实CNN2对细胞侵袭的影响，我们在SW620细胞系中增加CNN2的表达，并观察到侵袭增加以及相关蛋白表达水平升高。
结果
在癌组织中检测到CNN2的高表达水平。沉默CNN2可抑制细胞侵袭，下调N-钙黏蛋白（N-CA）和C- myc蛋白，并上调E-钙黏蛋白（E-CA），这表明CNN2促进结肠癌细胞侵袭。在SW620细胞系中增加CNN2水平则显示出相反的结果。
结论
CNN2可能起到促进结肠癌的作用。
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