Expression and correlation of , and in bladder transitional cell carcinoma.

BACKGROUND

To explore the expression and correlation of B-cell-specific Moloney murine leukemia virus insertion site 1 (), astrocyte elevated gene-1 () and fragile histidine triad () in bladder transitional cell carcinoma (BTCC).

METHODS

Forty-six tissue samples were obtained from patients diagnosed with primary bladder cancer during the first radical cystectomy between June 2010 and January 2014. The expression of , and in normal tissues and BTCC tissues in different histological cell types, clinical pathological stages and lymph node metastatic status were evaluated by quantitative real time polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry. Relationships between , and were determined using linear correlation coefficient.

RESULTS

The results of qRT-PCR showed the relative expression of and were higher and the expression of was lower in BTCC tissues than those in normal tissues, whether in different histological cell types, clinical pathological stages or lymph node metastatic status (P<0.05). Similarly, the up-regulated expression of and and down-regulated expression of in BTCC tissues were observed by the Western blot and immunohistochemistry compared with normal tissues (P<0.05). Linear correlation analysis showed the expression of was positively correlated with (r>0.90, P<0.01), which was negatively correlated with the expression of , respectively (r=-0.84, P<0.05).

CONCLUSIONS

The study demonstrated the up-regulated expression of and and the down-regulated expression of in BTCC tissues. The interaction of , and may involve in the tumorigenesis, progression and invasion of BTCC through NF-κB/MMP-9 pathway.