Su Wei, Li Chengwen, Li Xin
Department of Urology Surgery, Characteristic Medical Center of the Chinese People's Armed Police Force, Tianjin 300162, China.
Department of Urology Surgery, Tianjin Fourth Centre Hospital, Tianjin 300140, China.
Transl Cancer Res. 2020 Mar;9(3):1326-1336. doi: 10.21037/tcr.2020.01.13.
To explore the expression and correlation of B-cell-specific Moloney murine leukemia virus insertion site 1 (), astrocyte elevated gene-1 () and fragile histidine triad () in bladder transitional cell carcinoma (BTCC).
Forty-six tissue samples were obtained from patients diagnosed with primary bladder cancer during the first radical cystectomy between June 2010 and January 2014. The expression of , and in normal tissues and BTCC tissues in different histological cell types, clinical pathological stages and lymph node metastatic status were evaluated by quantitative real time polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry. Relationships between , and were determined using linear correlation coefficient.
The results of qRT-PCR showed the relative expression of and were higher and the expression of was lower in BTCC tissues than those in normal tissues, whether in different histological cell types, clinical pathological stages or lymph node metastatic status (P<0.05). Similarly, the up-regulated expression of and and down-regulated expression of in BTCC tissues were observed by the Western blot and immunohistochemistry compared with normal tissues (P<0.05). Linear correlation analysis showed the expression of was positively correlated with (r>0.90, P<0.01), which was negatively correlated with the expression of , respectively (r=-0.84, P<0.05).
The study demonstrated the up-regulated expression of and and the down-regulated expression of in BTCC tissues. The interaction of , and may involve in the tumorigenesis, progression and invasion of BTCC through NF-κB/MMP-9 pathway.
探讨B细胞特异性莫洛尼鼠白血病病毒插入位点1（）、星形胶质细胞上调基因-1（）和脆性组氨酸三联体（）在膀胱移行细胞癌（BTCC）中的表达及相关性。
收集2010年6月至2014年1月期间首次根治性膀胱切除术中确诊为原发性膀胱癌患者的46份组织样本。采用定量实时聚合酶链反应（qRT-PCR）、蛋白质免疫印迹法和免疫组织化学法评估正常组织和BTCC组织中不同组织学细胞类型、临床病理分期及淋巴结转移状态下、和的表达情况。利用线性相关系数确定、和之间的关系。
qRT-PCR结果显示，无论在不同组织学细胞类型、临床病理分期还是淋巴结转移状态下，BTCC组织中的相对表达量均高于正常组织，而的表达量低于正常组织（P<0.05）。同样，与正常组织相比，蛋白质免疫印迹法和免疫组织化学法观察到BTCC组织中表达上调，表达下调（P<0.05）。线性相关分析显示，的表达与呈正相关（r>0.90，P<0.01），与的表达呈负相关（r=-0.84，P<0.05）。
本研究表明BTCC组织中表达上调，表达下调。、和之间的相互作用可能通过NF-κB/MMP-9途径参与BTCC的发生、发展及侵袭过程。
