Kimman T G, Zimmer G M, Straver P J, de Leeuw P W
Am J Vet Res. 1986 Jan;47(1):143-7.
The potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (BRSV) was investigated. The technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. In 9 epizootics of respiratory tract disease, BRSV infection was diagnosed in 1 or more calves. The BRSV was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus was recovered by cell culture from 17 of these. In the 4 remaining calves, infectious bovine rhinotracheitis virus or parainfluenza type 3 virus was isolated. In calves with high levels of maternally derived antibodies, direct demonstration of the virus was found to be essential for making a diagnosis of BRSV. In 3 of the 9 epizootics, the majority of 4- to 8-month-old calves had a significant increase in antibody concentration against BRSV, whereas only a few 1- to 2-month-old calves present during the other 6 epizootics did so. However, at least in 4 of the 6 herds with younger calves, direct detection of the virus in 2 or more calves indicated that BRSV was involved in the epizootic. Cytologic examination of wash fluids from BRSV-infected animals identified an increased neutrophil/large mononuclear cell ratio. Occasionally, bronchiolar casts and syncytial giant cells with inclusion bodies were seen. In lung lavage fluids of 4 experimentally infected specific-pathogen-free calves, BRSV-containing cells were detected by immunofluorescence for 12 to 18 days after inoculation.
对肺灌洗术诊断和研究牛呼吸道合胞病毒（BRSV）感染的潜力进行了调查。该技术在野外条件下操作简便，即使是患有呼吸道疾病的犊牛，也未观察到严重的缺点。在9次呼吸道疾病流行中，在1头或多头犊牛中诊断出BRSV感染。通过免疫荧光法从灌洗回收的细胞中，在32头犊牛中的21头检测到了BRSV，其中17头通过细胞培养分离出了病毒。在其余4头犊牛中，分离出了传染性牛鼻气管炎病毒或3型副流感病毒。在母源抗体水平高的犊牛中，发现直接证明病毒对于诊断BRSV至关重要。在9次流行中的3次，4至8月龄犊牛的大多数针对BRSV的抗体浓度显著增加，而在其他6次流行中出现的1至2月龄犊牛中只有少数如此。然而，至少在有较年幼犊牛的6个牛群中的4个，在2头或更多犊牛中直接检测到病毒表明BRSV与该流行病有关。对BRSV感染动物的冲洗液进行细胞学检查，发现中性粒细胞/大单核细胞比例增加。偶尔可见细支气管铸型和带有包涵体的多核巨细胞。在4头实验感染的无特定病原体犊牛的肺灌洗液中，接种后12至18天通过免疫荧光法检测到含BRSV的细胞。
