细胞离心涂片法联合核固红苦味酸靛胭脂染色在无精子症男性隐匿性精子症检测中的作用
Role of Cytocentrifugation Combined with Nuclear Fast Picroindigocarmine Staining in Detecting Cryptozoospermia in Men Diagnosed with Azoospermia.
作者信息
Sharma Rakesh Kumar, Gupta Sajal, Agarwal Ashok, Finelli Renata, Kuroda Shinnosuke, Saleh Ramadan, Boitrelle Florence, Kavoussi Parviz, Gül Murat, Tadros Nicholas, Ko Edmund, Farkouh Ala'a, Henkel Ralf, Arafa Mohamed, Rambhatla Amarnath, Shah Rupin
机构信息
American Center for Reproductive Medicine, Cleveland, OH, USA.
Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University, Sohag, Egypt.
出版信息
World J Mens Health. 2022 Oct;40(4):627-635. doi: 10.5534/wjmh.210210. Epub 2022 Jan 19.
PURPOSE
Azoospermia is defined as the absence of spermatozoa in the pellet of a centrifuged semen sample. In fact, when a basic semen analysis fails to detect sperm in the ejaculate, there is still the possibility of detecting rare sperm after centrifugation of the sample and examination of the pellet. In this study, we assessed the role of Cytospin centrifugation in combination with the nuclear fast picroindigocarmine (NF-PIC) staining in identifying sperm in azoospermic samples.
MATERIALS AND METHODS
Semen samples of 251 men diagnosed as having azoospermia after standard examination were further analyzed by Cytospin centrifugation in combination with NF-PIC staining.
RESULTS
Sperm were detected in 60 men (23.9%), thus changing their diagnosis to cryptozoospermia.
CONCLUSIONS
By identifying sperm in the semen of men who were thought to have total azoospermia, the Cytospin NF-PIC test can alter the diagnosis and further treatment of these men.
目的
无精子症定义为离心后的精液样本沉淀中无精子。实际上，当基础精液分析未能在射精中检测到精子时，在对样本进行离心并检查沉淀后仍有可能检测到罕见精子。在本研究中，我们评估了细胞离心涂片法结合核固红苦味酸靛胭脂（NF-PIC）染色在鉴定无精子症样本中精子的作用。
材料与方法
对251名经标准检查诊断为无精子症的男性的精液样本，采用细胞离心涂片法结合NF-PIC染色进行进一步分析。
结果
在60名男性（23.9%）中检测到精子，从而将他们的诊断改为隐匿性精子症。
结论
通过在被认为患有完全无精子症的男性精液中鉴定精子，细胞离心涂片NF-PIC检测可以改变这些男性的诊断和进一步治疗方案。
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