大鼠血小板中针对原代培养大鼠肝细胞的两种生长抑制剂。

Two types of growth inhibitor in rat platelets for primary cultured rat hepatocytes.

作者信息

Nakamura T, Teramoto H, Tomita Y, Ichihara A

出版信息

Biochem Biophys Res Commun. 1986 Jan 29;134(2):755-63. doi: 10.1016/s0006-291x(86)80485-5.

PMID:3511909

Abstract

Rat platelets contain two types of growth inhibitor of adult rat hepatocytes in primary culture. One, named platelet derived growth inhibitor (PDGI)-alpha, is a heat- and acid-labile protein with a molecular weight of over 200 KD that is not released on thrombin treatment. The other, named PDGI-beta, is a heat- and acid-stable factor with a molecular weight of 24 KD that is released by thrombin. Both PDGI-alpha and -beta were inactivated by treatment with dithiothreitol. They both caused dose-dependent inhibition of DNA synthesis stimulated by insulin plus epidermal growth factor. These inhibitions were closely correlated with marked decrease in the labeling index. Neither PDGI-alpha nor -beta had a cytotoxic effect as judged by phase-contrast microscopic examination of the cells nor inhibition of protein synthesis. The properties of PDGI-beta suggest that it may be identical with transforming growth factor-beta. These results indicate that rat platelets contain not only a growth factor (HGF), but also growth inhibitors that affect adult rat hepatocytes.

摘要

大鼠血小板含有两种原代培养成年大鼠肝细胞生长抑制剂。一种名为血小板衍生生长抑制剂（PDGI）-α，是一种分子量超过200 KD的热不稳定和酸不稳定蛋白，经凝血酶处理后不会释放。另一种名为PDGI-β，是一种分子量为24 KD的热稳定和酸稳定因子，可被凝血酶释放。PDGI-α和-β经二硫苏糖醇处理后均失活。它们都能剂量依赖性地抑制胰岛素加表皮生长因子刺激的DNA合成。这些抑制作用与标记指数的显著降低密切相关。通过相差显微镜检查细胞以及对蛋白质合成的抑制判断，PDGI-α和-β均无细胞毒性作用。PDGI-β的特性表明它可能与转化生长因子-β相同。这些结果表明，大鼠血小板不仅含有一种生长因子（肝细胞生长因子），还含有影响成年大鼠肝细胞的生长抑制剂。