Arunrattiyakorn Panarat, Ponprateep Sirikwan, Kaennonsang Nirawan, Charapok Yoktip, Punphuet Yotwadee, Krajangsang Sukhumaporn, Tangteerawatana Piyatida, Limtrakul Anirut
Chemistry Department, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand.
Microbiology Department, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand.
J Appl Microbiol. 2022 Apr;132(4):2823-2831. doi: 10.1111/jam.15474. Epub 2022 Feb 16.
To isolate polystyrene-degrading bacteria from the gut of superworms and investigate their ability to degrade polystyrene (PS).
Three PS-degrading bacteria identified as Pseudomonas sp. EDB1, Bacillus sp. EDA4 and Brevibacterium sp. EDX were successfully isolated from the gut of superworms (Zophobas atratus Larvae) that ingest PS. Incubating PS with each strain for 30-day led to the formation of biofilm on the PS film. Scanning electron microscopy (SEM) revealed considerable damage (in terms of pits formation) on the surface of the PS films. FTIR analysis suggested the incorporation of carbonyl group into the carbon backbone of PS. Decreasing of WCA of microbial-treated PS film confirmed a chemical change from hydrophobicity to hydrophilicity on the PS surface. Based on these results, we conclude that all isolates had the ability to degrade PS.
Brevibacterium sp. EDX (GenBank MZ32399) was isolated as the most efficient PS-degrading strain based on the most changing in both PS surface morphology (SEM and WCA analyses) and chemical modification (FTIR analysis) in its PS degradation process.
This was the first study to describe the PS degradation by Brevibacterium sp. EDX, and thus provided for its development in the plastic remediation process.
从大麦虫肠道中分离聚苯乙烯降解菌，并研究其降解聚苯乙烯（PS）的能力。
从摄食PS的大麦虫（暗黑鳃金龟幼虫）肠道中成功分离出3株PS降解菌，分别鉴定为假单胞菌属EDB1、芽孢杆菌属EDA4和短杆菌属EDX。将PS与各菌株一起培养30天，导致PS膜上形成生物膜。扫描电子显微镜（SEM）显示PS膜表面有相当程度的损伤（表现为凹坑形成）。傅里叶变换红外光谱（FTIR）分析表明羰基已掺入PS的碳主链中。经微生物处理的PS膜的水接触角（WCA）降低，证实了PS表面从疏水性到亲水性的化学变化。基于这些结果，我们得出结论，所有分离株都具有降解PS的能力。
基于短杆菌属EDX在其PS降解过程中PS表面形态（SEM和WCA分析）和化学修饰（FTIR分析）变化最大，被分离为最有效的PS降解菌株。
这是首次描述短杆菌属EDX降解PS的研究，从而为其在塑料修复过程中的开发应用提供了依据。
