Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.
Best Pract Res Clin Endocrinol Metab. 2022 Jul;36(4):101609. doi: 10.1016/j.beem.2021.101609. Epub 2021 Dec 27.
For decades, anabolic androgenic agents have represented the substance class most frequently observed in doping control samples. They comprise synthetic and pseudoendogenous anabolic androgenic steroids and other, mostly non-steroidal compounds with (presumed) positive effects on muscle mass and function. While exogenous substances can easily be detected by gas/liquid chromatography and mass spectrometry, significantly more complex methodologies including the longitudinal monitoring of individual urinary steroid concentrations/ratios and isotope ratio mass spectrometry are required to provide evidence for the exogenous administration of endogenous compounds. This narrative review summarizes the efforts made within the last 5 years to further improve the detection of anabolic agents in doping control samples. Different approaches such as the identification of novel metabolites and biomarkers, the acquisition of complementary mass spectrometric data, and the development of new analytical strategies were employed to increase method sensitivity and retrospectivity while simultaneously reducing method complexity to facilitate a higher and faster sample throughput.
几十年来，合成代谢雄激素一直是兴奋剂检测样本中最常被观察到的物质类别。它们包括合成和假性内源性合成代谢雄激素类固醇，以及其他具有（假定）对肌肉质量和功能积极影响的非甾体化合物。虽然外源性物质可以很容易地通过气相/液相色谱和质谱法检测，但需要更复杂的方法，包括个体尿类固醇浓度/比值的纵向监测和同位素比质谱法，以提供内源性化合物外源性给药的证据。本综述总结了过去 5 年在进一步提高兴奋剂检测样本中合成代谢药物检测方面所做的努力。不同的方法，如鉴定新的代谢物和生物标志物，获取补充的质谱数据，以及开发新的分析策略，被用来提高方法的灵敏度和追溯性，同时降低方法的复杂性，以提高更高和更快的样品通量。
