Dluzen D E, Ramirez V D
Endocrinology. 1986 Mar;118(3):1110-3. doi: 10.1210/endo-118-3-1110.
We examined in vitro LHRH release as a function of time after ovariectomy (OVX). Adult female rats (diestrous day 1 and 11-16, 61-68, and more than 120 days post-OVX) were decapitated, and the suprachiasmatic-medial preoptic area-medial basal hypothalamus was removed and superfused in vitro. There was a statistically significant (P less than 0.05) 2-fold decrease in mean in vitro LHRH release 11-16 days post-OVX that returned to levels observed in diestrous day 1 females 60 days post-OVX. LHRH cycle amplitudes of 11- to 16-day ovariectomized rats were reduced and significantly lower (P less than 0.05) than those of more than 120-day ovariectomized rats. These results indicate a complex relationship between ovarian steroid hormone removal and LHRH pulse generator function that is only revealed after direct measurement of LHRH release.
我们研究了去卵巢（OVX）后不同时间点体外促黄体生成素释放激素（LHRH）的释放情况。成年雌性大鼠（动情间期第1天以及去卵巢后11 - 16天、61 - 68天和超过120天）被断头，取出视交叉上核 - 内侧视前区 - 下丘脑内侧基底部并进行体外灌流。去卵巢后11 - 16天，体外LHRH平均释放量有统计学意义的（P < 0.05）2倍下降，而去卵巢60天后又恢复到动情间期第1天雌性大鼠的水平。11至16天去卵巢大鼠的LHRH周期振幅降低，且显著低于（P < 0.05）超过120天去卵巢大鼠的LHRH周期振幅。这些结果表明卵巢甾体激素去除与LHRH脉冲发生器功能之间存在复杂关系，这种关系只有在直接测量LHRH释放后才能揭示。
