Ahmed C E, Dees W L, Ojeda S R
Endocrinology. 1986 Apr;118(4):1682-9. doi: 10.1210/endo-118-4-1682.
The nature and role of the peptidergic innervation of the ovary were examined by determining the location and function of vasoactive intestinal peptide (VIP)-containing nerve fibers in the immature rat ovary. Immunohistofluorescence analysis of prepubertal ovaries using a specific VIP antibody revealed sparse delicate VIP-immunoreactive fibers localized around veins and arterioles, in the interstitial tissue, and associated with the thecal layers of developing follicles. Radioimmunoassayable VIP content was found to be approximately 100 pg/ovary (3 nM). The VIP immunoreactivity coeluted with authentic VIP when subjected to Sephadex G-25 chromatography. VIP enhanced in vitro progesterone release from infantile (12 days old), juvenile (30 days old), and peripubertal ovaries and estradiol release during the two latter developmental periods. The maximal estradiol response to VIP occurred during the early and first proestrous phases of puberty. No response was observed during estrus or first diestrus. The progesterone response to VIP increased moderately between day 12 and first proestrus, and then strikingly at estrus and first diestrus. The stimulatory effect of VIP on ovarian steroid production was dose related, as determined in ovaries from PMSG-treated immature rats (ED50 = 215, 44, and 51 nM for estradiol, androgen, and progesterone, respectively). The specificity of the VIP effect was tested using five other gastrointestinal peptides (porcine peptide histidine isoleucine, gastric inhibitory polypeptide, secretin, motilin, and glucagon). Only peptide histidine isoleucine, which has the greatest sequence homology with VIP, enhanced ovarian steroid production at 50% of VIP effectiveness. VIPergic nerves thus appear to be involved in the developmental regulation of ovarian steroidogenesis.
通过确定未成熟大鼠卵巢中含血管活性肠肽（VIP）神经纤维的位置和功能，研究了卵巢肽能神经支配的性质和作用。使用特异性VIP抗体对青春期前卵巢进行免疫组织荧光分析，结果显示稀疏纤细的VIP免疫反应性纤维位于静脉和小动脉周围、间质组织中，并与发育卵泡的卵泡膜层相关。放射免疫分析法测得的VIP含量约为100 pg/卵巢（3 nM）。经Sephadex G - 25柱层析后，VIP免疫反应性与纯品VIP共洗脱。VIP可增强幼年（12日龄）、少年（30日龄）和青春期前卵巢的体外孕酮释放，并在青春期后两个发育阶段增强雌二醇释放。对VIP的最大雌二醇反应发生在青春期的早期和首次发情前期。发情期或首次间情期未观察到反应。对VIP的孕酮反应在第12天至首次发情前期适度增加，然后在发情期和首次间情期显著增加。如在经孕马血清促性腺激素（PMSG）处理的未成熟大鼠卵巢中所测定的，VIP对卵巢类固醇生成的刺激作用呈剂量依赖性（雌二醇、雄激素和孕酮的ED50分别为215、44和51 nM）。使用其他五种胃肠肽（猪肽组氨酸异亮氨酸、胃抑制多肽、促胰液素、胃动素和胰高血糖素）测试了VIP作用的特异性。只有与VIP序列同源性最高的肽组氨酸异亮氨酸在50%的VIP效能下增强了卵巢类固醇生成。因此，含VIP神经似乎参与了卵巢类固醇生成的发育调节。
