College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Int J Biol Macromol. 2022 Apr 15;204:50-61. doi: 10.1016/j.ijbiomac.2022.01.169. Epub 2022 Feb 2.
Two polysaccharides from Crocus sativus petals (PCSPs), PCSPA and PCSPB, presented immunopotentiatory activity through activating macrophages via MAPK and NF-κB pathway. In this study, two PCSPs were investigated for the adjuvant effects and underlying mechanisms using ovalbumin (OVA) in mice. PCSPA and PCSPB remarkably not only boosted the OVA-specific IgG antibody and its isotype titers, but strengthened splenocyte proliferation and natural killer cell activities in immunized mice. Both PCSPs also dramatically triggered the production of Th1- and Th2-cytokines and facilitated the gene expression of Th1- and Th2-cytokines and transcription factors in OVA-stimulated splenocytes. In mechanisms, two PCSPs rapidly elicited the gene and protein expression of cytokines and chemokines, promoted the recruitment and antigen uptake of immune cells in the injected-muscles, and augmented the migration and antigen transport of immune cells to the draining lymph nodes. These findings demonstrated that PCSPs enhanced and improved immune responses and simultaneously elicited Th1- and Th2-immune responses to OVA through activating innate immune microenvironment, and that they could act as promising vaccine adjuvant candidates.
藏红花花瓣中的两种多糖（PCSPs），PCSPA 和 PCSPB，通过 MAPK 和 NF-κB 途径激活巨噬细胞，呈现出免疫增强活性。在这项研究中，使用卵清蛋白（OVA）在小鼠中研究了两种 PCSP 的佐剂作用和潜在机制。PCSPA 和 PCSPB 不仅显著提高了 OVA 特异性 IgG 抗体及其同种型滴度，而且增强了免疫小鼠的脾细胞增殖和自然杀伤细胞活性。两种 PCSPs 还显著触发了 Th1 和 Th2 细胞因子的产生，并促进了 OVA 刺激的脾细胞中 Th1 和 Th2 细胞因子和转录因子的基因表达。在机制上，两种 PCSPs 迅速引发细胞因子和趋化因子的基因和蛋白表达，促进注射肌肉中免疫细胞的募集和抗原摄取，并增强免疫细胞向引流淋巴结的迁移和抗原运输。这些发现表明，PCSPs 通过激活先天免疫微环境增强和改善了对 OVA 的免疫反应，并同时引发了 Th1 和 Th2 免疫反应，它们可能是有前途的疫苗佐剂候选物。
