Yusupov M M, Spirin A S
FEBS Lett. 1986 Mar 3;197(1-2):229-33. doi: 10.1016/0014-5793(86)80332-5.
The hot tritium bombardment technique [(1976) Dokl. Akad. Nauk SSSR 228, 1237-1238] was used for studying the surface localization of ribosomal proteins on Escherichia coli ribosomes. The degree of tritium labeling of proteins was considered as a measure of their exposure (surface localization). Proteins S1, S4, S7, S9 and/or S11, S12 and/or L20, S13, S18, S20, S21, L5, L6, L7/L12, L10, L11, L16, L17, L24, L26 and L27 were shown to be the most exposed on the ribosome surface. The sets of exposed ribosomal proteins on the surface of 70 S ribosomes, on the one hand, and the surfaces of 50 S and 30 S ribosomal subunits in the dissociated state, on the other, were compared. It was found that the dissociation of ribosomes into subunits did not result in exposure of additional ribosomal proteins. The conclusion was drawn that proteins are absent from the contacting surfaces of the ribosomal subunits.
热氚轰击技术[（1976年）苏联科学院报告228，1237 - 1238]被用于研究核糖体蛋白在大肠杆菌核糖体上的表面定位。蛋白质的氚标记程度被视为其暴露程度（表面定位）的一种度量。结果表明，蛋白质S1、S4、S7、S9和/或S11、S12和/或L20、S13、S18、S20、S21、L5、L6、L7/L12、L10、L11、L16、L17、L24、L26和L27在核糖体表面的暴露程度最高。一方面比较了70S核糖体表面暴露的核糖体蛋白组，另一方面比较了解离状态下50S和30S核糖体亚基表面暴露的核糖体蛋白组。发现核糖体解离成亚基并不会导致额外的核糖体蛋白暴露。由此得出结论，核糖体亚基的接触面上不存在蛋白质。
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