Gedrovich A, Shishkov A, Goldanskii V, Baratova L, Grebenshchikov N, Efimov A
N.N. Semenov Institute of Chemical Physics, Academy of Sciences, Moscow, USSR.
Eur Biophys J. 1991;19(6):283-6. doi: 10.1007/BF00183316.
We propose the use of data on the topography of the label-accessible surface of a protein molecule obtained by the method of tritium planigraphy as a criterion for choosing the optimal intermediate arrangements of alpha-helices in globular proteins so as to model their three-dimensional structures. This approach has been used for modelling the three-dimensional structure of parvalbumin III from pike. The proposed model has been compared with high-resolution X-ray structural data for a related protein, paryvalbumin from carp. The possibilities and limitations of this approach are discussed.
我们建议将通过氚平面造影法获得的蛋白质分子标记可及表面的拓扑数据用作选择球状蛋白质中α-螺旋最佳中间排列的标准，以便对其三维结构进行建模。这种方法已用于对梭子鱼的小白蛋白III的三维结构进行建模。所提出的模型已与相关蛋白质——鲤鱼的小白蛋白的高分辨率X射线结构数据进行了比较。讨论了这种方法的可能性和局限性。
