伊朗鸡传染性贫血病毒（CIAV）的分子特征及首个全基因组测序
Molecular characterization and the first full sequencing genome of chicken infectious anemia virus (CIAV) in Iran.
作者信息
Hosseini H, Majidi S, Ziafati Kafi Z, Esmaeelzadeh Dizaji R, Ghalyanchilangeroudi A
机构信息
Department of Clinical Science, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
出版信息
Iran J Vet Res. 2021 Fall;22(4):331-336. doi: 10.22099/ijvr.2021.38455.5593.
BACKGROUND
The chicken infectious anemia virus (CIAV) is an important pathogen that causes severe immunosuppression in young chickens.
AIMS
The study aims to characterize the genotype and full-length sequencing of CIAV strains in Iran.
METHODS
First, the collected thymus samples were investigated by conventional PCR for CIAV detection. Second, one of the CIAV positive samples (UT-Zahraee) was chosen for full genome sequencing.
RESULTS
Throughout 2017, we detected 13 CIAVs isolated from 40 broiler flocks of different provinces of Iran. A comparison of the complete sequences of the genome and homologies of the nucleotides revealed that UT-Zahraee had a high similarity with American and Egyptian CIAV isolates. Moreover, sequence analysis showed that UT-Zahraee shared high homology with previously reported Iranian CIAV strains, Chinese, and Egyptian isolates.
CONCLUSION
This study is the first report of full genome sequencing of CIAV strain from Iran. It will be beneficial to understand better the epidemiology and molecular characteristics of CIAV circulating in Iran.
背景
鸡传染性贫血病毒（CIAV）是一种重要的病原体，可导致雏鸡严重免疫抑制。
目的
本研究旨在对伊朗CIAV毒株进行基因型鉴定和全基因组测序。
方法
首先，通过常规PCR对采集的胸腺样本进行CIAV检测。其次，选择其中一个CIAV阳性样本（UT-Zahraee）进行全基因组测序。
结果
在2017年期间，我们从伊朗不同省份的40个肉鸡群中检测到13株CIAV。对基因组的完整序列和核苷酸同源性进行比较后发现，UT-Zahraee与美国和埃及的CIAV分离株具有高度相似性。此外，序列分析表明，UT-Zahraee与先前报道的伊朗CIAV毒株、中国和埃及的分离株具有高度同源性。
结论
本研究是伊朗CIAV毒株全基因组测序的首次报道。这将有助于更好地了解伊朗流行的CIAV的流行病学和分子特征。
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