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在生物样品中由2-氧代戊二酸生成亮蓝色荧光团。

generation of a bright blue fluorophore from 2-oxoglutarate in biological samples.

作者信息

Kim Yumin, Kang Sangyoon, Lee Byung Hun, Song Youngjun, Kang Sunah, Park Hye Yoon, Lee Yan

机构信息

Department of Chemistry, College of Natural Sciences, Seoul National University Seoul 08826 Korea

Department of Physics and Astronomy, College of Natural Sciences, Seoul National University Seoul 08826 Korea

出版信息

Chem Sci. 2021 Dec 7;13(2):365-372. doi: 10.1039/d1sc05808h. eCollection 2022 Jan 5.

DOI:10.1039/d1sc05808h
PMID:35126969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8729799/
Abstract

We discovered the generation of a new bright blue fluorophore from a particular type of amine and 2-oxoglutarate (2-OG) under mild conditions without any chemical additives. Two β-aminoethylamine molecules and three 2-OG molecules form an unprecedented 2-pyridone structure with a fused γ-lactam ring (DTPP) complex reactions including double decarboxylation and quintuple dehydration. The DTPP fluorophore shows a high quantum yield (80%) and photostability. The great potential of the present DTPP generation in the quantitative analysis of 2-OG in biosamples is demonstrated.

摘要

我们发现在温和条件下且无需任何化学添加剂的情况下，一种特定类型的胺与2-氧代戊二酸（2-OG）可生成一种新的亮蓝色荧光团。两个β-氨基乙胺分子和三个2-OG分子通过包括双脱羧和五次脱水在内的复杂反应形成了一种前所未有的带有稠合γ-内酰胺环的2-吡啶酮结构（DTPP）。DTPP荧光团具有高量子产率（80%）和光稳定性。证明了当前DTPP生成法在生物样品中2-OG定量分析方面的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/666aeabfb509/d1sc05808h-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/fa7e8d0e9541/d1sc05808h-s1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/e0114a8793b5/d1sc05808h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/78ddde6235b1/d1sc05808h-s2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/76d0734a0b6f/d1sc05808h-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/666aeabfb509/d1sc05808h-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/fa7e8d0e9541/d1sc05808h-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/b420612a2cd2/d1sc05808h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/17858a80045f/d1sc05808h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/196aa2f50cb7/d1sc05808h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/e0114a8793b5/d1sc05808h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/78ddde6235b1/d1sc05808h-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/1ce3226c223e/d1sc05808h-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/76d0734a0b6f/d1sc05808h-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19a0/8729799/666aeabfb509/d1sc05808h-f7.jpg

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