Celio M R, Keller G A, Bloom F E
J Histochem Cytochem. 1986 Apr;34(4):491-500. doi: 10.1177/34.4.3512699.
We have utilized immunocryoultramicrotomy to detect synapsin, somatostatin, parvalbumin, and tubulin at the ultrastructural level. Immunocryoultramicrotomy combines informative identification of morphology with accurate immunolabeling. Moreover, since no detergents or organic solvents are used to enable antibody penetration, and since no enzyme marker diffusion occurs, localization of the antigens should be more accurate. Accordingly, it was possible to localize precisely all four antigens within a well-preserved structure. Application of this method has important advantages for high-resolution localization of molecules relevant to neuronal function.
我们利用免疫冷冻超薄切片术在超微结构水平检测突触素、生长抑素、小白蛋白和微管蛋白。免疫冷冻超薄切片术将形态学的信息识别与精确的免疫标记相结合。此外，由于不使用去污剂或有机溶剂来促进抗体渗透，且不存在酶标记扩散，抗原的定位应该更准确。因此，能够在保存良好的结构中精确地定位所有四种抗原。该方法的应用对于与神经元功能相关分子的高分辨率定位具有重要优势。
