从羧酸到腈肟的化学酶一锅法反应

Chemoenzymatic one-pot reaction from carboxylic acid to nitrile oxime.

作者信息

Horvat Melissa, Weilch Victoria, Rädisch Robert, Hecko Sebastian, Schiefer Astrid, Rudroff Florian, Wilding Birgit, Klempier Norbert, Pátek Miroslav, Martínková Ludmila, Winkler Margit

机构信息

Institute of Molecular Biotechnology, Graz University of Technology Petersgasse 14 A-8010 Graz Austria

Institute of Microbiology of the Czech Academy of Sciences Vídeňská 1083 CZ-142 20 Prague Czech Republic

出版信息

Catal Sci Technol. 2021 Nov 30;12(1):62-66. doi: 10.1039/d1cy01694f. eCollection 2022 Jan 4.

We report a new chemoenzymatic cascade starting with aldehyde synthesis by carboxylic acid reductase (CAR) followed by chemical oxime formation. The final step to the nitrile is catalyzed by aldoxime dehydratase (Oxd). Full conversions of phenylacetic acid and hexanoic acid were achieved in a two-phase mode.