Department of Ophthalmology, Visual and Anatomical Sciences, School of Medicine, Wayne State University, Detroit, MI, United States.
Front Cell Infect Microbiol. 2022 Jan 21;11:782063. doi: 10.3389/fcimb.2021.782063. eCollection 2021.
To test the effects of acidic . neutral pH glycyrrhizin (GLY) on the unwounded and wounded normal mouse cornea and after infection with isolates KEI 1025 and multidrug-resistant MDR9.
Acidic or neutral GLY . phosphate-buffered saline (PBS) was topically applied to normal or wounded corneas of C57BL/6 mice. In unwounded corneas, goblet cells and corneal nerves were stained and quantitated. After wounding, corneas were fluorescein stained and photographed using a slit lamp. Mice also were infected with KEI 1025 or MDR9 and the protective effects of GLY pH evaluated comparatively.
In the unwounded cornea, application of acidic or neutral GLY . PBS reduced the number of bulbar conjunctival goblet cells but did not alter corneal nerve density. Similar application of GLY to scarified corneas delayed wound closure. After KEI 1025 infection, none of the GLY . PBS-treated corneas perforated; GLY treatment also decreased plate count (neutral pH more effective) and reduced MPO and several cytokines. Similarly, for MDR9, GLY at either pH was protective and also enhanced the effects of moxifloxacin to which MDR9 is resistant.
Acidic or neutral pH GLY decreased goblet cell number but had no effect on nerve density. After corneal wounding, GLY at either pH (1) delayed wound closure and, (2) after infection, decreased keratitis when used alone or in combination with moxifloxacin. Neutral pH did not alter the therapeutic effect of GLY and would be preferred if used clinically.
检测酸性、中性 pH 甘草酸（GLY）对未受伤和受伤正常小鼠角膜的影响，以及对 KEI1025 株和多药耐药 MDR9 株感染后的影响。
将酸性或中性 GLY、磷酸盐缓冲盐水（PBS）局部应用于 C57BL/6 小鼠正常或受伤的角膜。在未受伤的角膜中，对杯状细胞和角膜神经进行染色和定量。在受伤后，用荧光素对角膜进行染色，并使用裂隙灯拍摄照片。还对小鼠进行了 KEI1025 或 MDR9 感染，并比较评估 GLY pH 的保护作用。
在未受伤的角膜中，应用酸性或中性 GLY、PBS 减少了球结膜杯状细胞的数量，但不改变角膜神经密度。在划伤的角膜上应用类似的 GLY 会延迟伤口愈合。在 KEI1025 感染后，没有一个 GLY、PBS 处理的角膜穿孔；GLY 处理还降低了平板计数（中性 pH 更有效），并减少了 MPO 和几种细胞因子。同样，对于 MDR9，两种 pH 的 GLY 都具有保护作用，并且还增强了对 MDR9 耐药的莫西沙星的作用。
酸性或中性 pH 的 GLY 减少了杯状细胞数量，但对神经密度没有影响。在角膜受伤后，两种 pH 的 GLY（1）延迟了伤口愈合，（2）在感染后，单独使用或与莫西沙星联合使用时，减少了角膜炎。中性 pH 不会改变 GLY 的治疗效果，如果在临床上使用，会更受青睐。
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