鉴定电鳐和大鼠之间保守的一种突触小泡抗原（分子量86,000）。

Identification of a synaptic vesicle antigen (Mr 86,000) conserved between Torpedo and rat.

作者信息

Walker J H, Kristjansson G I, Stadler H

出版信息

J Neurochem. 1986 Mar;46(3):875-81. doi: 10.1111/j.1471-4159.1986.tb13053.x.

DOI:10.1111/j.1471-4159.1986.tb13053.x

Abstract

Antisera were raised in guinea pigs to synaptic vesicles purified from the electric organ of Torpedo marmorata. In cholinergic nerve terminals from Torpedo the major antigens identified had Mr 300,000-150,000, 86,000, and 18,000. The Mr 86,000 antigen was conserved between Torpedo and rat, where it is neuron-specific and concentrated in nerve terminals. When rat brain synaptosomes are subfractionated the antigen is associated with synaptic vesicles. The antigen is not found in the cytoskeleton and in the vesicle-free cytosol. Immunohistochemical localization of the antigen in rat shows it to be associated with synapses in diaphragm, cerebellum, hippocampus, and cerebral cortex. The staining pattern of the antigen indicates that the antigen is not cholinergic-specific. The function of the Mr 86,000 antigen remains to be identified.

摘要

用从电鳐电器官中纯化的突触小泡免疫豚鼠制备抗血清。在电鳐的胆碱能神经末梢中，鉴定出的主要抗原有分子量为300,000 - 150,000、86,000和18,000的蛋白。分子量为86,000的抗原在电鳐和大鼠之间保守，在大鼠中它是神经元特异性的且集中在神经末梢。当对大鼠脑突触体进行亚分级分离时，该抗原与突触小泡相关。在细胞骨架和无小泡的胞质溶胶中未发现该抗原。该抗原在大鼠中的免疫组织化学定位显示它与膈肌、小脑、海马体和大脑皮层中的突触相关。该抗原的染色模式表明它不是胆碱能特异性的。分子量为86,000的抗原的功能仍有待确定。