Koster J J, Boustead C M, Middleton C A, Walker J H
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
Biochem J. 1993 Apr 15;291 ( Pt 2)(Pt 2):595-600. doi: 10.1042/bj2910595.
The Ca(2+)- and phospholipid-binding protein, annexin V, has been shown by an immune assay to represent 0.4% of total cell protein in cultured chick-embryo fibroblasts. Immunofluorescent localization studies indicate that in primary cultures the protein is abundant in the cytoplasm of the cells and also extends into the nucleus. Nuclear staining is no longer detectable, however, in approx. 25% of the cells following sub-culture. Sub-populations of annexin V are associated with cytoskeletal structures and with the inner face of the plasma membrane in a Ca(2+)-independent manner. In addition, we report results indicating the secretion of annexin V from this cell type.
钙和磷脂结合蛋白膜联蛋白V，通过免疫测定法显示在培养的鸡胚成纤维细胞中占总细胞蛋白的0.4%。免疫荧光定位研究表明，在原代培养物中，该蛋白在细胞的细胞质中含量丰富，并且还延伸到细胞核中。然而，在传代培养后，约25%的细胞中不再能检测到核染色。膜联蛋白V的亚群以不依赖钙的方式与细胞骨架结构和质膜内表面相关联。此外，我们报告的结果表明这种细胞类型会分泌膜联蛋白V。
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