State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, China.
Department of Rehabilitation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, China.
Biomed Res Int. 2022 Jan 28;2022:8647483. doi: 10.1155/2022/8647483. eCollection 2022.
LI09 could protect rats from D-galactosamine- (D-GalN-) induced liver injury. However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood. The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D-GalN-induced rat liver injury. Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity. Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL-5, and MIP-3 were determined in the nonsevere cohort than the severe cohort. The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts. The intestinal microbiota composition was different between the two cohorts. Ten phylotypes assigned to , Clostridia_UCG-014, Lachnospiraceae, Lachnospiraceae_NK4A136, and were closely associated with the nonsevere cohort, among which, ASV8_Lachnospiraceae_NK4A136 was the most associated one. At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts. Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort. In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, , and , were associated with the better protective effect of LI09 against D-GalN-induced rat liver injury. They were likely to enhance the effectiveness of LI09, and their clinical application deserves further investigation.
LI09 可保护大鼠免受半乳糖胺（D-GalN）诱导的肝损伤。然而，LI09 对肝损伤的保护作用的个体差异仍知之甚少。本研究旨在确定与 LI09 对 D-GalN 诱导的大鼠肝损伤更好的保护作用相关的多种肠道细菌。根据肝损伤严重程度，将两个大鼠队列（非严重和严重队列）分为两组。非严重队列的 ALB 水平较高，ALT、AST、TBA、TB、IL-5 和 MIP-3 水平较低。非严重和严重队列的肠道微生物群的 alpha 多样性指数（即观察到的物种、香农和皮耶罗指数）没有显著差异。两组肠道微生物群的组成不同。10 个分类群分配到 、Clostridia_UCG-014、Lachnospiraceae、Lachnospiraceae_NK4A136 和 与非严重队列密切相关，其中 ASV8_Lachnospiraceae_NK4A136 是最相关的一个。在结构水平上，确定了两组与两个队列肠道微生物群网络最相关的分类群。其中，ASV135_Lachnospiraceae_NK4A136 是非严重队列微生物群网络中最强大的守门员。总之，一些肠道细菌，如 Lachnospiraceae_NK4A136、、和 ，与 LI09 对 D-GalN 诱导的大鼠肝损伤的更好保护作用相关。它们可能增强了 LI09 的有效性，其临床应用值得进一步研究。
