State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310000, China.
School of Biological Sciences, The University of Auckland, Auckland, New Zealand.
BMC Microbiol. 2020 Jun 5;20(1):144. doi: 10.1186/s12866-020-01827-2.
Probiotics are effective to rectify the imbalanced gut microbiota in the diseased cohorts. Two Bifidobacterium strains (LI09 and LI10) were found to alleviate D-galactosamine-induced liver damage (LD) in rats in our previous work. A series of bioinformatic and statistical analyses were performed to determine the vital bacteria in the gut microbiotas altered by the LI09 or LI10 in rats.
Two groups of representative phylotypes could distinguish the gut microbiotas of LI09 or LI10 groups from the other groups. Among them, OTU170_Porphyromonadaceae acted as a gatekeeper in LI09 group, while OTU12_Bacteroides was determined with multiple correlations in the gut network of LI10 group. Multiple reduced OTUs associated with LC and increased OTUs associated with health were determined in LI09 or LI10 groups, among which, increased OTU51_Barnesiella and reduced OTU99_Barnesiella could be associated with the protective effects of both the two probiotics. The gut microbiotas in LI09, LI10 and positive control groups were clustered into three clusters, i.e., Cluster_1_Microbiota, Cluster_2_Microbiota and Cluster_3_Microbiota, by Partition Around Medoids clustering analysis. Cluster_2_Microbiota was determined at least dysbiotic status due to its greatest LD dysbiosis ratio, lowest levels of liver function variables and plasma cytokines compared with the two other clustered microbiotas, suggesting the treated rats in Cluster_2 were at better health status.
Our findings suggest that OTU170_Porphyromonadaceae and OTU12_Bacteroides are vital in the gut microbiotas altered by LI09 and LI10. Characteristics of the LD cohorts treated by LI09 or LI10 at different gut microbial colonization states could help monitor the cohorts' health status.
益生菌可有效纠正患病队列中失衡的肠道微生物群。在我们之前的工作中，发现两种双歧杆菌（LI09 和 LI10）可缓解大鼠半乳糖胺诱导的肝损伤（LD）。进行了一系列生物信息学和统计分析，以确定 LI09 或 LI10 改变大鼠肠道微生物群的重要细菌。
两组有代表性的分类群可将 LI09 或 LI10 组的肠道微生物群与其他组区分开来。其中，OTU170_Porphyromonadaceae 在 LI09 组中充当守门员，而 OTU12_Bacteroides 在 LI10 组的肠道网络中与多个相关因素相关。在 LI09 或 LI10 组中确定了与 LC 相关的多个减少的 OTU 和与健康相关的多个增加的 OTU，其中，增加的 OTU51_Barnesiella 和减少的 OTU99_Barnesiella 可能与这两种益生菌的保护作用有关。通过 Partition Around Medoids 聚类分析，LI09、LI10 和阳性对照组的肠道微生物群聚类为三个群，即群 1 微生物群、群 2 微生物群和群 3 微生物群。由于其最大的 LD 失调比、最低的肝功能变量和血浆细胞因子水平与其他两个聚类微生物群相比，群 2 微生物群被确定至少处于失调状态，这表明处于群 2 中的治疗大鼠处于更好的健康状态。
我们的研究结果表明，OTU170_Porphyromonadaceae 和 OTU12_Bacteroides 是 LI09 和 LI10 改变的肠道微生物群中的重要细菌。LI09 或 LI10 处理的 LD 队列在不同肠道微生物定植状态下的特征可帮助监测队列的健康状况。
