水飞蓟素通过 Nrf2 保护大鼠对抗百草枯诱导的急性肾损伤。
Silymarin protects the rats against paraquat-induced acute kidney injury via Nrf2.
机构信息
Department of Emergency, 585241Xingtai People's Hospital of Hebei Province, Xingtai, China.
出版信息
Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221074334. doi: 10.1177/09603271221074334.
BACKGROUND
Paraquat (PQ) poisoning induces severe acute kidney injury and causes extremely high rate of death. In this study, we aimed to investigate the protective effects of silymarin on PQ-induced acute kidney injury and explore the underlying mechanisms.
METHODS
A rat model was established through intraperitoneal injection of PQ. Rats were administrated with saline or silymarin for 3 days. Then, survival rate, physiological parameters, and renal injury score were evaluated. The apoptosis and oxidative stress in kidney tissues were determined through hematoxylin and eosin staining, quantitative reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assays.
RESULTS
Silymarin administration could significantly increase the survival rate of PQ-poisoned rats. It was found that silymarin treatment improved renal function, decreased injury score in kidney tissues, and inhibited the apoptosis and oxidative stress in PQ-induced acute kidney injury through the activating the signaling pathway of Nrf2 and promoting its nuclear translocation.
CONCLUSION
Silymarin exhibited a protective effect against PQ-induced kidney injury, suggesting that treatment with this flavonoid could be a potential therapeutic agent for the treatment of acute kidney injury.
背景
百草枯（PQ）中毒会导致严重的急性肾损伤，并导致极高的死亡率。在本研究中，我们旨在探讨水飞蓟素对 PQ 诱导的急性肾损伤的保护作用，并探讨其潜在机制。
方法
通过腹腔注射 PQ 建立大鼠模型。大鼠给予生理盐水或水飞蓟素治疗 3 天。然后，评估存活率、生理参数和肾损伤评分。通过苏木精和伊红染色、定量逆转录聚合酶链反应、Western blot 和酶联免疫吸附试验测定肾组织中的细胞凋亡和氧化应激。
结果
水飞蓟素给药可显著提高 PQ 中毒大鼠的存活率。研究发现，水飞蓟素治疗通过激活 Nrf2 信号通路并促进其核转位，改善了肾功能，降低了肾组织损伤评分，并抑制了 PQ 诱导的急性肾损伤中的细胞凋亡和氧化应激。
结论
水飞蓟素对 PQ 诱导的肾损伤具有保护作用，提示这种黄酮类化合物的治疗可能是急性肾损伤治疗的一种潜在治疗剂。
Zotero 插件