Szewczuk A, Milnerowicz H, Sobiech K A
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):565-6.
Incubation time of human kidney gamma-glutamyl-transferase (GGT) with specific antiserum and precipitate formation caused no effect on inhibition of the enzyme activity. In the obtained precipitate GGT activity was independent of the amount of antiserum and contained 30% of the initial enzyme activity. After reaction of the serum with human liver GGT or with the urine enzyme a precipitate formation and decrease in the enzyme activity was noted. In the serum incubated with GGT isolated from animal kidneys, neither inhibition nor precipitation was observed. The precipitate of bovine kidney GGT with a specific antibody independently of the amount of antibody contained 50% of activity. After treatment of bovine GGT with bromelain four protein fractions giving precipitin lines in crossed-immunoelectrophoresis were observed but only one of them preserved catalytic activity. Immunogenicity of newly obtained immobilized GGT preparations was much lower than that of the native enzyme. A distinct and different influence of antibody on the affinity of native and immobilized GGT preparations to donor and acceptors of gamma-glutamyl group was noted. The immunofluorescent technique was used for GGT localization in some bovine tissue sections.
人肾γ-谷氨酰转移酶（GGT）与特异性抗血清的孵育时间以及沉淀形成对酶活性的抑制没有影响。在所获得的沉淀物中，GGT活性与抗血清的量无关，且含有初始酶活性的30%。血清与人肝GGT或尿酶反应后，观察到有沉淀形成且酶活性降低。在与从动物肾脏分离的GGT孵育的血清中，未观察到抑制或沉淀现象。牛肾GGT与特异性抗体形成的沉淀物，无论抗体量多少，均含有50%的活性。用菠萝蛋白酶处理牛GGT后，在交叉免疫电泳中观察到四个产生沉淀素线的蛋白质组分，但其中只有一个保留催化活性。新获得的固定化GGT制剂的免疫原性远低于天然酶。注意到抗体对天然和固定化GGT制剂与γ-谷氨酰基团供体和受体亲和力有明显不同的影响。免疫荧光技术用于在一些牛组织切片中定位GGT。
