Ben Saad Marwa, Ben Said Myriam, Bousselmi Latifa, Ghrabi Ahmed
Laboratory of wastewater and Environment Water Researches and Technologies Center, CERTE, Soliman, BP, Tunisia.
National Agronomic Institute of Tunisia, University of Carthage, Mahrajène, Tunis, Tunisia.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2022;57(2):111-116. doi: 10.1080/10934529.2022.2036551. Epub 2022 Feb 6.
The aim of this study was to enhance the rhizobacterium potential in horizontal subsurface flow constructed wetland (CW) system planted by using specific and lytic phages. The bioinoculation of specific bacteriophage for target bacteria; , and the monitoring of bacterial inactivation under different conditions showed the effectiveness of this methodology to enhance bacteria reduction and consequentially ameliorate purification performance of this studied biological treatment system. The injection of the phage at a concentration equal to 10 UFP/mL within the rhizosphere of the inoculated filter (F) was allowed 1 U-Log of improvement of bacterial inactivation compared to the control filter (T) nearly 1 logarithmic unit thus, a 90% improvement of bacteria reduction. When we increased the phage titer (10 UFP/mL), the bacterial reduction equal to 2.75 U-Log (N/N) was registered that corresponds to a decrease of nearly 99.9%. According to the first-order model, the inactivation coefficient is equal to 2.29 min (0.88 min for the first experiment) and the bacterial reduction rate is 5 times higher than that determined for the control filter. This results show the positive impact of the phage in the bacterial inactivation and the improvement of water treatment of the biofilter C.
本研究的目的是通过使用特异性裂解噬菌体来增强水平潜流人工湿地（CW）系统中根际细菌的潜力。对目标细菌接种特异性噬菌体，并监测不同条件下细菌的失活情况，结果表明该方法对于提高细菌去除率进而改善所研究生物处理系统的净化性能是有效的。在接种过滤器（F）的根际中以10噬菌斑形成单位/毫升的浓度注入噬菌体，与对照过滤器（T）相比，细菌失活提高了1个对数单位，细菌去除率提高了90%。当我们提高噬菌体滴度（10噬菌斑形成单位/毫升）时，细菌去除率达到2.75个对数单位（N/N），相当于减少了近99.9%。根据一级模型，失活系数为2.29分钟（第一次实验为0.88分钟），细菌去除率比对照过滤器高5倍。这些结果表明噬菌体对细菌失活以及生物滤池C的水处理改善具有积极影响。
