Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology.
Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology;
J Vis Exp. 2022 Jan 19(179). doi: 10.3791/63257.
Acute myocardial infarction is a common cardiovascular disease with high mortality. Myocardial reperfusion injury can counteract the beneficial effects of heart reflow and induce secondary myocardial injury. A simple and reproducible model of myocardial infarction and myocardial ischemia-reperfusion injury is a good tool for researchers. Here, a customizable method to create a myocardial infarction (MI) model and MIRI by precision ligation of the left anterior descending coronary artery (LAD) through micromanipulation is described. Accurate and reproducible ligature positioning of the LAD helps obtain consistent results for heart injury. ST-segment changes can help to identify model accuracy. The serum level of cardiac troponin T (cTnT) is used to assess the myocardial injury, cardiac ultrasound is employed to evaluate the myocardial systolic function, and Evans-Blue/triphenyl tetrazolium chloride staining is used to measure infarct size. In general, this protocol reduces procedure duration, ensures controllable infarct size, and improves mouse survival.
急性心肌梗死是一种常见的心血管疾病，死亡率较高。心肌再灌注损伤会抵消心脏再灌注的有益作用，并引发继发性心肌损伤。一个简单且可重现的心肌梗死和心肌缺血再灌注损伤模型是研究人员的良好工具。本文描述了一种通过微操作精确结扎左前降支冠状动脉（LAD）来创建心肌梗死（MI）模型和 MIRI 的可定制方法。LAD 结扎的精确和可重现定位有助于获得一致的心脏损伤结果。ST 段变化有助于确定模型的准确性。血清心肌肌钙蛋白 T（cTnT）水平用于评估心肌损伤，心脏超声用于评估心肌收缩功能， Evans-Blue/三苯基四氮唑氯化物染色用于测量梗死面积。总的来说，该方案缩短了手术时间，确保了可控制的梗死面积，并提高了小鼠的存活率。
