Kuro Atsuyuki, Morimoto Naoki, Hara Tomoya, Matsuoka Yuki, Fukui Michika, Hihara Masakatsu, Kusumoto Kenji, Kakudo Natsuko
Department of Plastic and Reconstructive Surgery, Kansai Medical University Medical Center, Moriguchi, Japan.
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Med Mol Morphol. 2022 Jun;55(2):91-99. doi: 10.1007/s00795-021-00310-9. Epub 2022 Feb 7.
Supercooling preservation below 0 °C allows the storage of the transplantable sources in an unfrozen state. This can improve the safety and efficacy of storage by improving the inhibition of metabolism and organ preservation in comparison with conventional preservation at 4 °C. We have developed a supercooling technique using a voltage-applied apparatus without perfusion. We examined the preservation effects of our supercooling preservation technique in a rat model of artery transplantation. Our technique produces a supercooled state at - 2 °C with application of 1000 V. The viability of tissue cells from rat arteries was found to be higher with storage using the proposed method than that under ordinary conditions. Damage to the vascular endothelium of the femoral artery preserved by voltage-applied supercooling at - 2 °C was reduced compared to storage under ordinary conditions. Artery graft revival was successfully achieved with graft patency after supercooling preservation, and 1 week outcomes for post-transplanted grafts, including thrombosis, were better with supercooling preservation than with conventional 4 °C preservation. Supercooling artery preservation at - 2 °C with 1000 V promises to greatly prolong preservation time and improve post-transplant outcomes.
0°C以下的过冷保存可使可移植源在未冻结状态下储存。与4°C的传统保存相比，这可以通过改善代谢抑制和器官保存来提高保存的安全性和有效性。我们开发了一种不进行灌注的使用施加电压装置的过冷技术。我们在大鼠动脉移植模型中研究了我们的过冷保存技术的保存效果。我们的技术在施加1000V电压时可在-2°C产生过冷状态。发现使用所提出的方法储存时，大鼠动脉组织细胞的活力高于普通条件下的活力。与普通条件下储存相比，在-2°C通过施加电压过冷保存的股动脉血管内皮损伤减少。过冷保存后成功实现了动脉移植物再通，移植后移植物1周的结果，包括血栓形成，过冷保存比传统的4°C保存更好。在-2°C施加1000V进行过冷动脉保存有望大大延长保存时间并改善移植后结果。
