School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.
School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China.
Mikrochim Acta. 2022 Feb 7;189(3):87. doi: 10.1007/s00604-022-05196-7.
A novel nanoassembly was constructed through encapsulating upconversion nanoparticles (UCNPs) into a metal-organic framework structure (ZIF-8), in which doxorubicin (DOX) was absorbed into pores of ZIF-8. The blue emission of UCNPs was quenched by DOX through the fluorescence resonance energy transfer (FRET) strategy. When the nanoprobe was exposed to food samples with different pH values, ZIF-8 collapsed to release DOX molecules, resulting in upconversion recovery. The porous structure of ZIF-8 provides abundant space for DOX absorption, which significantly improves the detection capacities and accuracy. It is shown that the probe has a good linear relationship when pH values vary from 2.5 to 7.4, and can distinguish pH variations as low as 0.5 in real samples. This strategy has been successfully used to determine food spoilage by determination of pH variations.
通过将上转换纳米粒子（UCNPs）封装到金属有机骨架结构（ZIF-8）中，构建了一种新型纳米组装体，其中阿霉素（DOX）被吸收到 ZIF-8 的孔中。通过荧光共振能量转移（FRET）策略，UCNPs 的蓝色发射被 DOX 猝灭。当纳米探针暴露于具有不同 pH 值的食物样品中时，ZIF-8 会崩溃以释放 DOX 分子，从而导致上转换恢复。ZIF-8 的多孔结构为 DOX 的吸收提供了丰富的空间，从而显著提高了检测能力和准确性。结果表明，该探针在 pH 值从 2.5 到 7.4 变化时具有良好的线性关系，并且可以在实际样品中区分低至 0.5 的 pH 值变化。该策略已成功用于通过测定 pH 值变化来确定食物变质。
